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Ros measurement kit

Manufactured by Nanjing Jiancheng
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Sourced in China

The ROS measurement kit is a laboratory equipment designed to measure reactive oxygen species (ROS) in various samples. The kit provides the necessary tools and reagents to perform ROS quantification, without making any claims about its intended use or application.

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Lab products found in correlation

Facscalibur, by Beckman Coulter (1 mentions) Fluorescence microscope, by Olympus (1 mentions) Fc500mcl mpl, by Beckman Coulter (1 mentions)

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ROS kit (21 citations) Measurement kits (5 citations)

2 protocols using ros measurement kit

1

Intracellular ROS Detection Protocol

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The concentration of intracellular ROS was detected using an ROS measurement kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer's instructions and visualized under a fluorescence microscope (Olympus, Tokyo, Japan). The fluorescence was monitored using a FACS Calibur flow cytometer (FC500 MCL/MPL, Beckman Coulter Inc., Brea, CA) with excitation at 488 nm and emission at 525 nm. ROS generation was quantified by the mean fluorescence intensity over that of the control.
Yan Y., Chen X., Huang J., Huan C, & Li C. (2022). H2O2-induced oxidative stress impairs meat quality by inducing apoptosis and autophagy via ROS/NF-κB signaling pathway in broiler thigh muscle. Poultry Science, 101(4), 101759.
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2

ROS Measurement using Flow Cytometry

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ROS measurements were using an ROS-measurement kit (Nanjing Jiancheng Bioengineering Institute) followed the methods described by Song et al. (2012). (link) Briefly, the fresh samples were used to prepare single-cell suspensions for flow cytometry. Then the flow cytometer (FC500 MCL/MPL, Beckman Coulter Inc., CA) with the excitation at 488 nm and emission at 525 nm were used to monitor the formation of 2′7′-dichloro-fluorescein (DCF). ROS generation was quantified by the mean fluorescence intensity of the CON group.
Chang Q., Cai H., Wei L, & Lan R. (2021). Chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers. Poultry Science, 101(1), 101515.
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