Lactate dehydrogenase ldh cytotoxicity assay kit

Sodium arsenite (NaAsO₂), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), gallic acid, rutin, penicillin/streptomycin, hematoxylin, eosin, and protease inhibitor were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM), and other cell culture reagents were obtained from Gibco (Carlsbad, CA, USA). Dimethyl sulfoxide (DMSO) was from Bioshop (Canada). RNA extraction kits were purchased from RiboEx and Hybrid-R (Gene All, South Korea). Tissue protein extraction (T-PER), cDNA synthesis (ReverTra Ace qPCR RT Kit), and BCA protein assay kits were purchased from Thermo Scientific (Waltham, Massachusetts, USA). SYBR green qPCR kit was purchased from TOYOBO (Japan). Primary antibodies (phospho-ERK1/2, phospho-JNK phospho p38, Bax, Bcl-2, cleaved caspase-3, and caspase-3) and β-actin were purchased from Cell Signaling, Danvers, MA, USA. Secondary antibody (goat anti-rabbit IgG-HRP) was purchased from Santa Cruz (Santa Cruz, CA, USA). ECL detection kit was acquired from Abfrontire (South Korea), and the ALT and AST kits were from ASAN (South Korea). Lactate dehydrogenase (LDH) cytotoxicity assay kit was obtained from TAKARA (Japan), and the ROS detection kit was from Promega (USA). Zoletil 50 was supplied by Virbac S.A. (France).

Polyphenolic compounds, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT), 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) disodium salt (ABTS), fluorometric intracellular ROS kit, penicillin/streptomycin, and 2-(4-amidinophenyl)-6-indolecarbami dine dihydrochloride (DAPI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Parishin derivatives were bought from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, Sichuan, China). Fetal bovine serum (FBS) was obtained from Young in Frontier Company (Seoul, Korea). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was bought from MedChemExpress (Princeton, NJ, USA). Ham’s F-12 Nutrient Mix medium, BCA protein assay kit, and cDNA synthesis kit (ReverTra Ace qPCR RT Kit) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Eagle’s Minimum Essential Medium (EMEM) was obtained from ATCC (Manassas, VA, USA). H₂O₂ was purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). The SYBR green qPCR Kit was supplied by TOYOBO (Osaka, Japan). Lactate dehydrogenase (LDH) cytotoxicity assay kit was bought from TAKARA (Shiga Prefecture, Japan). Clarity western chemiluminescent (ECL) substrate kit was supplied by Bio-Rad Laboratories (Hercules, CA, USA).