The total weight of the reaction mixture was 1,670 mg and it contained 50 mg of the cellulosic/hemicellulosic sample, 50 mg enzyme mixture (a 1:1 ratio of a mixture of Celluclast 1.5 L and Novozyme 188, both of which were obtained from Sigma-Aldrich) and 50 mM citrate buffer (pH 5.2). The enzymatic hydrolysis was performed at 45°C in an orbital shaker set at 170 rpm (Ecotron incubator shaker, Infors). The samples (10 μl) were collected before enzyme addition (0 h), and after 2 h, 48 h and 72 h of enzymatic hydrolysis.
Ecotron incubator shaker
Manufactured by Infors
The Ecotron incubator shaker is a laboratory equipment designed for incubation and shaking of samples. It provides a controlled environment for cell culture, microbiology, and other applications that require temperature and agitation control.
Automatically generated - may contain errors
2 protocols using ecotron incubator shaker
1
Enzymatic Hydrolysis of Cellulosic Biomass
2
Enzymatic Saccharification of Wood Biomass
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Portions of 50 mg of dry wood powder (particle size 100–500 μm) from each of four biological replicates per line each with three technical replicates were used for enzymatic saccharification before and after acid pretreatment as described previously (Gandla et al., 2021 (link)). Pretreatment was performed using a single‐mode microwave system (Initiator Exp, Biotage, Uppsala, Sweden). Each reaction mixtures with pretreated or non‐pretreated material contained 5 mg liquid enzyme preparation (Cellic CTec‐2 obtained from Sigma‐Aldrich, St. Louis, MO, U.S.A.). Reaction mixtures were incubated for 72 h at 45°C and 170 rpm (Ecotron incubator shaker, Infors, Bottmingen, Swizerland). GPR was determined after 2 h using a glucometer (Accu‐Chek® Aviva; Roche Diagnostics Scandinavia AB, Bromma, Sweden), and monosaccharide sugar yields were determined after 72 h using a high‐performance anion‐exchange chromatography (HPAEC) system (ICS 5000, Dionex, Sunnyvale, CA) and standard monosaccharide solutions (Wang et al., 2018 ).
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