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2 protocols using cay10583

1

Characterization of Leukocyte Receptor Interactions

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Mouse anti-human CD56 and CD3 antibodies and 7AAD were purchased from BD Biosciences (Mississauga, ON, Canada). FITC-conjugated goat anti-rabbit IgG (GAR-FITC) and DTAF-conjugated streptavidin (SA-FITC) were from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). Polyclonal rabbit anti-human BLT1R and BLT2R antibodies, LTB4, CAY10583, U75302, and LY255283 were from Cayman Chemical (Ann Arbor, MI, USA). isotype control rabbit IgG was from InterSciences (Markham, ON, Canada). Biotinylated mouse anti-human BLTR antibody and isotype control were from AbD SeroTec (Raleigh, NC, USA). Human IL-2 and IL-15 were purchased from PeproTech (Dollard des Ormeaux, QC, Canada). MIP-1α was from Abcam (Cambridge, MA, USA). All other chemical agents were obtained from Sigma-Aldrich (Oakville, ON, Canada) unless otherwise mentioned.
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2

Inflammatory Mediators and Inhibitors

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Aspirin, celecoxib, and actinomycin d were purchased from Sigma Aldrich. Heparin was obtained from Fuso Pharmaceutical Industries. Ozagrel, 12-HHT, LY255283, and CAY10583 were purchased from Cayman Chemical Company. Methyl cellulose 400 was purchased from Wako Pure Chemical Industries. Recombinant human TNF was obtained from PeproTech. The human TNF-neutralizing antibody Infliximab was purchased from Janssen Biotech. The mouse TNF-neutralizing antibody D2H4 was purchased from Cell Signaling Technology. MMP-9 inhibitor I (an MMP-9–specific reagent) and MMP inhibitor II (a broad MMP inhibitor) were purchased from EMD Millipore.
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