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Taqman based chemistry

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TaqMan based chemistry is a real-time PCR (Polymerase Chain Reaction) technology developed by Thermo Fisher Scientific. It utilizes specific DNA probes labeled with fluorescent dyes to detect and quantify target DNA sequences during the amplification process. The core function of TaqMan chemistry is to provide a sensitive and accurate method for the detection and quantification of nucleic acid targets.

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Lab products found in correlation

Steponeplus real time pcr system, by Thermo Fisher Scientific (2 mentions) Lightcycler 480 sybr green 1 master mix, by Roche (2 mentions) Rneasy micro kit, by Qiagen (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Taqman probe assay, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

TaqMan chemistry (50 citations) TaqMan probe-based chemistry (7 citations) TaqMan chemistry-based miRNA assay system (5 citations)

2 protocols using taqman based chemistry

1

Quantitative Real-Time PCR Analysis

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Total RNA was purified using the RNeasy Micro Kit (Qiagen) and cDNA was prepared using the high capacity cDNA reverse transcription kit (Applied Biosystems). Real-time qPCR was performed with the real-time PCR StepOnePlus system (Applied Biosystems). Nr1d1 (Mm00520708_m1), Hk2 (Mm00443385_m1), Hmgcs (Mm01304569_m1) and Xbp1 (Mm00457358_m1) were detected with commercial Taqman probe assays (Applied Biosystems). Remaining assays were performed either with TaqMan based chemistry (Applied Bio Systems) or with LightCycler 480 SYBR Green I Master Mix (Roche), using the primers and probes detailed in Supplementary Table 2.
Penny H.A., Domingues R.G., Krauss M.Z., Melo-Gonzalez F., Lawson M.A., Dickson S., Parkinson J., Hurry M., Purse C., Jegham E., Godinho-Silva C., Rendas M., Veiga-Fernandes H., Bechtold D.A., Grencis R.K., Toellner K.M., Waisman A., Swann J.R., Gibbs J.E, & Hepworth M.R. (2022). Rhythmicity of Intestinal IgA Responses Confers Oscillatory Commensal Microbiota Mutualism. Science immunology, 7(75), eabk2541.
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2

Quantitative Real-Time PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was purified using the RNeasy Micro Kit (Qiagen) and cDNA was prepared using the high capacity cDNA reverse transcription kit (Applied Biosystems). Real-time qPCR was performed with the real-time PCR StepOnePlus system (Applied Biosystems). Nr1d1 (Mm00520708_m1), Hk2 (Mm00443385_m1), Hmgcs (Mm01304569_m1) and Xbp1 (Mm00457358_m1) were detected with commercial Taqman probe assays (Applied Biosystems). Remaining assays were performed either with TaqMan based chemistry (Applied Bio Systems) or with LightCycler 480 SYBR Green I Master Mix (Roche), using the primers and probes detailed in Supplementary Table 2.
Penny H.A., Domingues R.G., Krauss M.Z., Melo-Gonzalez F., Lawson M.A., Dickson S., Parkinson J., Hurry M., Purse C., Jegham E., Godinho-Silva C., Rendas M., Veiga-Fernandes H., Bechtold D.A., Grencis R.K., Toellner K.M., Waisman A., Swann J.R., Gibbs J.E, & Hepworth M.R. (2022). Rhythmicity of Intestinal IgA Responses Confers Oscillatory Commensal Microbiota Mutualism. Science immunology, 7(75), eabk2541.
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