Lysozyme lys

Fibrinogen (Fib.), fraction I from bovine plasma, lysozyme (Lys.), and mucin (Muc.) from bovine submaxillary gland were purchased from Shanghai Yuanye Biotechnology Co. Ltd. Bovine serum albumin (BSA), copper(i) bromide (99%), copper(ii) bromide (99%) and Me₄Cyclam (98%) were obtained from Sigma-Aldrich. N-(3-Sulfopropyl)-N-(methacryloxyethyl)-N,N-dimethylammoniumbetaine (SBMA, 99%), 2-hydroxy-2-methylpropiophenone (HMPP, 99%) were purchased from J&k. ω-Mercaptoundecyl bromoisobutyrate (MUBiB, ≥95%) was obtained from Shanghai D&B Biological Science Technology Co. Ltd. N,N-Methylenebisacrylamide (Bis, 98%) was purchased from Sinopharm Chemical Reagent Co. Ltd. 3-Acrylamidophenylboronic acid (PBA, 98%) was obtained from Ark Pharm. Acrylamide (AM, 98.5%) was purchased from Xilong Chemical Industry. Sulfuric acid (H₂SO₄, AR), hydrogen peroxide (H₂O₂, 30% aqueous solution), ethanol (C₂H₅OH, AR), dimethyl sulfoxide (DMSO, AR), glucose (C₆H₁₂0₆, AR), sodium phosphate dibasic dodecahydrate (Na₂HPO₄, AR), and potassium dihydrogen phosphate (KH₂PO₄, AR) were purchased from Beijing Chemical Factory. Water used in the experiments was purified using a Millipore water purification system (UPR-II-10TNZP, Sichuan Youpu Ultrapure Technology Co. Ltd.). The saliva was collected from volunteers.

Ammonium hydroxide solution (27%, W/V), FeCl₃·6H₂O, FeSO₄·7H₂O, N,N,N′,N′-tetramethylenediamine (TEMED), absolute ethanol, acetic acid (HAc) were bought from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Ovalbumin (OVA), bovine serum albumin (BSA), bovine hemoglobin (BHb), cytochrome C (Cyt C) and lysozyme (Lys) were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). Allyl chloroacetate and N,N-methylenebisacrylamide (MBAA) were purchased from Aladdin chemistry Co., Ltd. (Shanghai, China). Vinyltriethoxysilane (VTEO) was obtained from Adamas Reagent Co., Ltd. (Shanghai, China). N-(3-aminopropyl)-imidazole was gained from TCI Development Co., Ltd. (Shanghai, China). Hydrazine hydrate and ammonium persulfate (APS) were bought from the Institute of Fucheng Chemicals (Tianjin, China). Sodium dodecyl sulfate (SDS) was supplied by Xilong chemistry Co., Ltd. (Shantou, China). All reagents were of analytical grade. All solutions were prepared using deionized water (18.25 MΩ cm, 25 °C). Phosphate buffer solutions (20 mmol L⁻¹, pH = 7.1) were used in this work for most experiments.