Human lung adenocarcinoma A549 (RCB0098) and human normal lung fibroblast WI-38 cells (RCB0702) were obtained from the RIKEN Bioresource Center, Ibaraki, Japan. Human lung epidermoid carcinoma Calu-1 was purchased from the Cell Lines Service (CLS; Eppelheim, Germany) and human lung large cell carcinoma COR-L23 from the European Collection of Authenticated Cell Cultures (ECACC; Salisbury, UK). A549 and WI-38 cells were cultured in D-MEM medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). Calu-1 and COR-L23 were cultured in RPMI-1640 medium (Gibco). These culture media were supplemented with 10% (v/v) fetal bovine serum (FBS; Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) and 1% antibiotics (Gibco) at 37°C in a 5% CO2 humidified atmosphere. All of the cell lines are mycoplasma free. The continuous cell lines are routinely checked in every other month by a PCR method using a service from The Center for Veterinary Diagnosis, Faculty of Veterinary Science, Mahidol University Salaya Campus, Nakorn Pathom, Thailand.
Calu 1
Manufactured by Cytion
Sourced in Germany
The Calu-1 is a compact and versatile laboratory equipment designed for basic analytical tasks. It features a user-friendly interface and essential functionalities to support general laboratory operations.
Automatically generated - may contain errors
Lab products found in correlation
Cor l23, by European Collection of Authenticated Cell Cultures (1 mentions)
Calu 1, by Thermo Fisher Scientific (1 mentions)
Wi 38 cells, by RIKEN BioResource Center (1 mentions)
Wi 38, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Hepg2, by Cytion (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
2 protocols using calu 1
1
Cell Culture Protocols for Lung Cancer
2
Cell Culture Protocols for Cancer Lines
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Human lung epidermoid carcinoma cells (Calu-1) and human hepatocellular carcinoma cells (HepG2) were obtained from the Cell Lines Service (CLS; Eppelheim, Germany), the American Type Tissue Culture Collection (ATCC, USA), and ScienCell (Carlsbad, CA, USA). Cell lines were maintained using protocols previously reported in the literature [89 (link)]. Briefly, Calu-1 and HepG2 cells were incubated at 37 °C and 5% CO2 in Eagle’s minimum essential medium (EMEM, Quality Biological, Gaithersburg, MD, USA) enriched with fetal bovine serum (FBS; Biowest, South America). To avoid the proliferation of pathogens, 1% penicillin/streptomycin (Gibco, Grand Island, NY, USA) was added.
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