Tapestation 4200 with high sensitivity d1000 screentape

Genomic DNA Library was synthesized from genomic DNA using a SMART-Seq HT kit (Takara Bio USA, Mountain View, CA, USA) and the Nextera XT DNA Library Kit (Illumina, San Diego, CA, USA), following the manufacturer’s standard protocol. The library quality was further evaluated using the TapeStation 4200 with High Sensitivity D1000 ScreenTape (Agilent Technologies, Santa Clara, CA). The library was quantified using the HS Qubit dsDNA assay (Thermo Fisher Scientific, Waltham, MA, USA).

The DNase I-treated or Poly(A) capture full-length double-stranded sequencing libraries were prepared using the Nextera XT DNA Library Kit (Illumina, San Diego, CA, USA) for Illumina sequencing following the manufacturer’s instructions, as described previously^{32 (link)}. The library quality was further analyzed using the TapeStation 4200 with High Sensitivity D1000 ScreenTape (Agilent Technologies, Santa Clara, CA, USA). All libraries were quantified using the HS Qubit dsDNA assay (Thermo Fisher Scientific, Waltham, MA, USA). All libraries were sequenced (2 × 75 bp) using Illumina NextSeq 500 (Illumina, San Diego, CA, USA). The FASTQ files were generated using the bcl2fastq software (Illumina, San Diego, CA, USA). The FASTQ data (GSE192928) are deposited in the Gene Expression Omnibus (GEO) (https://www.ncbi.nlm.nih.gov/geo/).

Total RNA Library was synthesized from total RNA using a SMART-Seq HT kit (Takara Bio USA, Mountain View, CA, USA) and the Nextera XT DNA Library Kit (Illumina, San Diego, CA, USA), following the manufacturer’s standard protocol. The library quality was further evaluated using the TapeStation 4200 with High Sensitivity D1000 ScreenTape (Agilent Technologies, Santa Clara, CA). The library was quantified using the HS Qubit dsDNA assay (Thermo Fisher Scientific, Waltham, MA, USA).