The name and source of the cell lines used in this study are: ES8 (St. Jude Children’s Research Hospital [SJCRH], RRID:CVCL_1204), A673-shEF (Francisco J Alonso [24 (link)], RRID:CVCL_JM58), JN-DSCRT (SJCRH, RRID:CVCL_9W68), EW8 (SJCRH, RRID:CVCL_V618), CADO-ES1 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures [DSMZ], RRID:CVCL_1103), RH30 (SJCRH, RRID:CVCL_0041), ES1 (SJCRH, RRID:CVCL_1198), H-EMC-SS (European Collection of Authenticated Cell Cultures, RRID:CVCL_1238), SaOS2 (SJCRH, RRID: CVCL_0548), CY143B (SJCRH, RRID:CVCL_2270), SJSA1 (SJCRH, RRID:CVCL_1697), RH3 (SJCRH, RRID:CVCL_L415), U2OS (SJCRH, RRID:CVCL_0042), SU-CCS-1 (ATCC, RRID:CVCL_B470), CHLA-258 (Children’s Oncology Group Cell Culture/Xenograft Repository, RRID:CVCL_A058), EW18 (Dr. Zisis Kozlakidis and International Agency for Research on Cancer/World Health Organization [IARC], RRID:CVCL_1213), EW13 (IARC, RRID:CVCL_1211), EW11 (IARC, RRID:CVCL_1209). Cell lines were authenticated using short tandem repeat analysis via PowerPlex (Promega) and tested for mycoplasma using MycoAlert (Lonza). Translocation status was confirmed using PCR and Fluorescence In Situ Hybridization (FISH).
Saos2
Manufactured by European Collection of Authenticated Cell Cultures
Sourced in United Kingdom
SaOS2 is a human osteosarcoma cell line derived from the primary tumor of an 11-year-old Caucasian female. It is a widely used model for the study of bone biology and osteogenic differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Powerplex, by Promega (1 mentions)
Mycoalert, by Lonza (1 mentions)
Panc 1, by European Collection of Authenticated Cell Cultures (1 mentions)
Mrc 5, by European Collection of Authenticated Cell Cultures (1 mentions)
Molt 4, by European Collection of Authenticated Cell Cultures (1 mentions)
Mcf 7, by European Collection of Authenticated Cell Cultures (1 mentions)
A2780, by European Collection of Authenticated Cell Cultures (1 mentions)
2 protocols using saos2
1
Detailed Cell Line Characterization
2
Characterization of Human Cell Lines
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Selected human tumour and non-tumour cell lines Jurkat (acute T cell leukemia), MOLT-4 (acute lymphoblastic leukemia), A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), PANC-1 (pancreas epithelioid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), SAOS-2 (osteosarcoma) and MRC-5 (non-tumour lung fibroblasts) were purchased from European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK) and cultured according to the provider's culture method guidelines. All cell lines were maintained at 37 °C in a humidified 5% carbon dioxide and 95% air incubator. The cells in low passage number (non-tumour primary cell line MRC-5 was used for a maximum of 10 passages and cancer cell lines were used for a maximum of 20 passages) and in an exponential growth phase were used for this study.
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