Fe2 so4 3

Manufactured by Merck Group

Sourced in United States

Fe2(SO4)3 is an inorganic chemical compound consisting of ferric ions (Fe3+) and sulfate ions (SO4^2-). It is a common laboratory reagent used in various scientific applications.

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Fe2(SO4)3·7H2O (3 citations) Ferric sulfate Fe2(SO4)3 (2 citations)

7 protocols using fe2 so4 3

Leptospirillum Oxidative Stress Response

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Two liter of Leptospirillum CF-1 culture was grown until late exponential phase. Cells were harvested by centrifugation at 9,000 × g for 20 min at 15°C. Then cells were resuspended in 25 mL of fresh medium and shaken for 30 min at 37°C. The cells were collected by centrifugation and washed with 10 mM H₂SO₄ and resuspended in 25 mL of fresh medium supplemented independently with either 260 mM Fe₂(SO₄)₃ (Sigma–Aldrich), 1 mM H₂O₂ (Merck), 6 mM K₂CrO₄ (Merck), or 4 mM diamide [1,1′-azobis(N,N-dimethylformamide; Sigma–Aldrich)] as oxidative agent for 1 h at 37°C. Samples treated with cobalamin were pre-incubated with 5 nM of cyanocobalamin (Sigma–Aldrich) for 1 h at 37°C in darkness. cyanocobalamin is by definition vitamin B₁₂, and represents the form mainly manufactured by industry (Martens et al., 2002 (link)).

Ferrer A., Rivera J., Zapata C., Norambuena J., Sandoval Á., Chávez R., Orellana O, & Levicán G. (2016). Cobalamin Protection against Oxidative Stress in the Acidophilic Iron-oxidizing Bacterium Leptospirillum Group II CF-1. Frontiers in Microbiology, 7, 748.

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Zebrafish Iron-Induced Retinal Damage

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Zebrafish were intravitreally injected with 0.5 μl of either FeSO₄ (Sigma-Aldrich; St. Louis, MO) or Fe₂(SO₄)₃ (Sigma-Aldrich; St. Louis, MO) at 0.5 mM in water using a 2.5 μl syringe with a 33-gauge rounded needle (Hamilton; Reno, NV). Treated fish were then placed in a dark incubator maintained at 32°C until the time of sample collection or 96 hours post injection (hpi), when they were placed back in standard light-dark conditions until the time of sample collection. Both 0.1 mM and 0.25 mM injections were also tested, however 0.5 mM was chosen for this study due to greater consistency and extent of damage as previously described (Shu et al., 2020 (link)). Fish requiring EdU injections were intraperitoneally injected with 50 μl of 1 mg/ml EdU (Life Technologies; Carlsbad, CA) using a 30-gauge needle every 12 hours beginning at 24 hours post iron injection (hpii) until a final injection at 96 hpii before being placed in recovery.

Boyd P, & Hyde D.R. (2022). Iron Contributes to Photoreceptor Degeneration and Müller Glia Proliferation in the Zebrafish Light-Treated Retina. Experimental eye research, 216, 108947.

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Culturing Candida albicans in LoFlo medium

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C. albicans strains were grown in LoFlo_glu medium. This medium contains 0.079% complete supplement mixture (CSM; MP Biomedicals), 0.19% yeast nitrogen base without amino acids and lacking folic acid and riboflavin (Formedium), 0.5% ammonium sulfate (Fluka), and 2% glucose. The pH was adapted to 5.5 in liquid medium. For some experiments, the growth medium of the wild-type strain SC5314 was supplemented with different concentrations of FeCl₃ (Sigma), Fe₂(SO₄)₃ (Sigma), BPS (Sigma), ferrozine (Sigma), or dibutyryl-cAMP (Sigma).

Demuyser L., Palmans I., Vandecruys P, & Van Dijck P. (2020). Molecular Elucidation of Riboflavin Production and Regulation in Candida albicans, toward a Novel Antifungal Drug Target. mSphere, 5(4), e00714-20.

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Aβ-induced inflammatory pathway

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PGE₂, PGD₂, Fe₂(SO₄)₃ and the inhibitors including AH6809 and GW9662 were obtained from Sigma-Aldrich Corp (St. Louis, MO, USA). M-30 was also obtained from Sigma-Aldrich Corp (St. Louis, MO, USA). 15d-PGJ₂ were obtained from Enzo Life International Inc. (Plymouth Meeting, PA). NS398 was purchased from Tangchao Chemical Co., Ltd. (Xi’an, China). Aβ was synthesized by Qiangyao Biotechnology (Shanghai, China). mPGES-1, EP2, DP1, Tf, TfR or scramble siRNA and antibody specific for Tf or TfR were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). COX-2 or L-PGDS cDNA plasmids were obtained from Origene Technologies (Rockville, MD, USA), and subcloned to the pCMV6-XL vector. Antibodies against β-actin, PS1 and Aβ (Stock No. 15126) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). mPGES-1 and L-PGDS antibody was obtained from Sigma-Aldrich Corp (St. Louis, MO, USA). Antibody specific for APH-1α/1β was purchased from Merck Millipore (Bedford, MA, USA). All reagents for the qRT-PCR and SDS-PAGE experiments were purchased from Bio-Rad Laboratories. All other reagents were from Invitrogen (Carlsbad, CA, USA) unless otherwise specified.

Lu C.D., Ma J.K., Luo Z.Y., Tai Q.X., Wang P, & Guan P.P. (2018). Transferrin is responsible for mediating the effects of iron ions on the regulation of anterior pharynx-defective-1α/β and Presenilin 1 expression via PGE2 and PGD2 at the early stage of Alzheimer’s Disease. Aging (Albany NY), 10(11), 3117-3135.

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Synthesis of PEDOT:Xanthan Gum Dispersions

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PEDOT:xanthan
gum dispersions with 1, 2, and 3% solid content and
at different PEDOT:xanthan gum ratios were synthesized by oxidative
polymerization according to the following procedure. In a 25 mL round-bottom
flask containing 19.6 mL of Milli-Q water, 0.3, 0.27, 0.2, or 0.13
g of xanthan gum (Acros) was dissolved and purged with N₂. Afterward, 0.1, 0.13, 0.2, or 0.27 g of EDOT monomer (Acros), together
with the 1.5 equiv of ammonium persulfate ((NH₄)₂S₂O_8, Sigma) and a catalytic amount (5 mg)
of iron(III) sulfate (Fe₂(SO₄)₃ Sigma)
was added, respectively. The reaction solution was kept at 0 °C
until completion (144 h). The dispersions were purified by dialysis
with Milli-Q water and a regenerated cellulose dialysis membrane (12–14
kD) from SpectrumLabs. Finally, dispersions containing different PEDOT:xanthan
gum ratios were obtained by varying the initial amount of grams (0.25:0.75,
0.33:0.67, 0.5:0.5, and 0.67:0.33).

del Agua I., Marina S., Pitsalidis C., Mantione D., Ferro M., Iandolo D., Sanchez-Sanchez A., Malliaras G.G., Owens R.M, & Mecerreyes D. (2018). Conducting Polymer Scaffolds Based on Poly(3,4-ethylenedioxythiophene) and Xanthan Gum for Live-Cell Monitoring. ACS Omega, 3(7), 7424-7431.

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Cation Exchange in Nafion Membranes

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In this study, Nafion ® 212 membranes were purchased in protonated (H + ) form from Ion Power Inc. (New Castle, DE) with the nominal thickness of 50 µm. Membranes were soaked into 0.5M (Li2SO4, Na2SO4, K2SO4, Cs2SO4, Fe2(SO4)3 and 1M (MgSO4, CuSO4, ZnSO4, FeSO4) aqueous sulfate solutions (from Sigma Aldrich and J.T. Baker) for 48 to 72 hours under ambient conditions to achieve new cationic forms, using the same procedure as described in a previous study. 4 The size of the membrane specimens, the volume of salt solutions and the amount of soak time were carefully selected to make sure that protons in membranes were fully exchanged with cations, as described previously. 71 Then, the membrane specimens were rinsed in deionized water for 3 times to remove excess solution on surface and stored in deionized water until use.
The membranes were dried in vacuum oven at 60C for at least 12 hours before testing.

Shi S ., Liu Z ., Lin Q ., Chen X , & Kusoglu A . (2020). Role of ionic interactions in the deformation and fracture behavior of perfluorosulfonic-acid membranes. Soft matter, 16(6).

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Synthesis of Iron Oxide Nanoparticles

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Typically, a solution containing 500 mg of PVP (MW = 40,000, Sigma-Aldrich) and 5.0 ml of n-pentanol (99%, Sigma-Aldrich) was prepared in a 10 ml glass vial. A 140 μl aqueous solution of 40 mg ml⁻¹ Fe₂(SO₄)₃ (Sigma-Aldrich) and a 500 μl of anhydrous ethanol (Pharmco-Aaper) were added into the glass vial and mixed thoroughly under vortex stirrer. The mixed solution was kept standing for 1 min to release gas bubbles. Then, a 60 μl aqueous solution of NH₃ (28 wt%, Sigma-Aldrich) and a 50 μl of TEOS (98%, Sigma-Aldrich) were added into the solution in sequence. After each addition, the mixture was gently shaken for 30 s. The mixture was incubated at 25 °C for 4 h to allow the reaction complete.

Yang Y., Chen G., Thanneeru S., He J., Liu K, & Nie Z. (2018). Synthesis and assembly of colloidal cuboids with tunable shape biaxiality. Nature Communications, 9, 4513.

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