Hs578t

The human breast cell line MCF-10 A, human breast cancer cell lines (MCF7, T47D, SKBR-3, MDA-MB-231 and Hs578T), human monocyte cell line THP-1 and human umbilical vein endothelial cell line HUVEC were all purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). T47D, SKBR3, MCF7, MDA-MB-231, Hs578T and HUVEC cells were cultured with DMEM medium (Procell, China) containing 10% fetal bovine serum (FBS). THP-1 cells were cultured with RPMI-1640 medium (Procell, China) with 10% FBS and 0.05 mM ß-Mercaptoethanol. MCF-10 A cells cultured with MEGM kit (Lonza Clonetics, Switzerland). All cells were cultured at 37 °C and in 5% CO₂ humidified atmosphere incubator.

Luminal A type BC cell line MCF-7 and two TNBC cell lines MDA-MB-231 and Hs578T were purchased from Procell Life Science &Technology Co.,Ltd. and iCell Bioscience Inc. (China). Cells were maintained at 37°C in a 5% CO₂-humidified incubator (Heal Force, China). MCF-7 cells were cultured in Minimum Essential Medium (Gibco Life Technologies, USA) containing 10% fetal bovine serum (FBS, Every Green, China), MDA-MB-231 cells in Leibovitz’s L-15 Medium (Procell) containing 10% FBS and Hs578T in Dulbecco’s modified eagle medium (Gibco Life Technologies) containing 10% FBS (Every Green).

The TNBC cell lines (Hs578T, BT549, MDA-MB-231, MDA-MB-157 and MDA-MB-468), human immortalized mammary epithelial cell line (MCF10A), and 293 T cells were purchased from Procell Life Science & Technology (Wuhan, China). MDA-MB-231, MDA-MB-157, MDA-MB-468, Hs578T, BT549, and 293 T cells were cultured in DMEM (Gibco, USA) medium containing 10% FBS (Wisent, Canada). MCF10A cells were grown in complete DMEM/F12 medium (Procell Life Science & Technology). All cells were maintained at 37 °C with 5% CO₂.