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Mvi sirna

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The MVI siRNA is a laboratory equipment product designed for RNA interference (RNAi) experiments. It is a tool for the efficient delivery and silencing of target genes in cell lines and primary cells. The core function of the MVI siRNA is to facilitate the introduction of small interfering RNA (siRNA) into cells, enabling the specific knockdown of gene expression.

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Lab products found in correlation

Incucyte live cell imager, by Sartorius (2 mentions) Scrambled sirna, by Qiagen (2 mentions) Incucyte software, by Sartorius (1 mentions)

2 protocols using mvi sirna

1

Cell Density Monitoring by Live Imaging

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Cells were seeded onto 96-well tissue culture dishes at equal densities in six replicates. After attachment over-night, cells were transfected with MVI siRNA, or scrambled siRNA (Qiagen). Photomicrographs were taken every hour using an IncuCyte live-cell imager (Essen Biosciences, Ann Harbour, MI) and confluency of cultures was measured using IncuCyte software. Confluency values between wells were normalised to initial confluency for comparison.
Hari-Gupta Y., Fili N., dos Santos Á., Cook A.W., Gough R.E., Reed H.C., Wang L., Aaron J., Venit T., Wait E., Grosse-Berkenbusch A., Gebhardt J.C., Percipalle P., Chew T.L., Martin-Fernandez M, & Toseland C.P. (2022). Myosin VI regulates the spatial organisation of mammalian transcription initiation. Nature Communications, 13, 1346.
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2

Measuring Cell Confluency Dynamics

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Cells were seeded onto 96-well tissue culture dishes at equal densities in 6 replicates. After attachment overnight, cells were transfected with MVI siRNA, or scrambled siRNA (Qiagen). Photomicrographs were taken every hour using an IncuCyte live cell imager (Essen Biosciences, Ann Harbor, MI) and confluency of cultures was measured using IncuCyte software. Confluency values between wells were normalised to initial confluency for comparison.
Hari‐Gupta Y., Fili N., Santos Á.d., Cook A.W., Gough R.E., Reed H., Wang L., Aaron J., Venit T., Wait E., Große-Berkenbusch A., Rief M., Percipalle P., Chew T., Martin-Fernandez M.L., & Toseland C.P. (2020). Nuclear myosin VI regulates the spatial organization of mammalian transcription initiation.
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