Dylight 488 goat anti mouse igg h l

Rabbit anti-TfR1 (Abcam). Mouse monoclonal anti-p-Tyr (PY20) (Santa Cruz Biotechnology). Rabbit anti-Src (Cell Signaling Technology). Rabbit anti-pAPN (ABclonal Biotechnology). Mouse monoclonal antibodies to His, HA, and GFP (CMCTAG, Milwaukee, USA). Mouse monoclonal anti-PEDV N protein was purchased from Medgene labs (FACS, 1:100; IF, 1:200; Western-blot, 1:1000). FITC-conjugated anti-PEDV polyclonal antibody was purchased from VMRD (1:200, PC-IFA-PEDV). Dylight 488 goat anti-mouse IgG (H+L) and Dylight 649 goat anti-rabbit IgG (H+L) were from MultiSciences (Lianke) Biotech, CO., LTD. Rabbit monoclonal anti-GAPDH and goat anti-rabbit IgG-HRP were from Bioworld Technology Inc (St. Louis Park, MN, USA). HRP-conjugated goat anti-mouse IgG (H+L) (Vazyme, Nanjing, China). Anti-rabbit IgG antibody (Beyotime).

Tumor tissues were embedded in optimal cutting temperature compound (Tissue-Tek, California, United States) and frozen. Ten μm cryosections were made for histological analysis following standard protocols. Immunofluorescence staining was performed as previously described^{22 (link)}. Briefly, each section was fixed in ice-cold acetone, incubated with blocking buffer (2% bovine serum albumin in PBS containing 5% donkey serum with 0.1% Triton X-100) at room temperature for 1 h, and then incubated with primary antibodies overnight at 4˚ C. On the second day, the sections were washed three times with PBS and incubated with secondary antibodies in the dark at room temperature. After 1 h, sections were mounted with DAPI (Abcam). A BX53-DP80 immunofluorescence microscope (Olympus) was used for analysis of staining. The following primary and secondary antibodies were used: cleaved-Caspase3 (CST, #9664), Ki67 (Abcam, #15580), DyLight488 goat anti-mouse IgG(H+L) (Multi Science, Zhejiang, China), and DyLight594 goat anti-rabbit IgG(H+L) (Multi Science).