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Fluorescent 700 800 secondary antibodies

Manufactured by Thermo Fisher Scientific
Sourced in United States

Fluorescent-700/800 secondary antibodies are laboratory reagents used for detection and visualization of target proteins in various biological applications. These antibodies are conjugated with fluorescent dyes that emit light in the near-infrared range of 700 or 800 nanometers, allowing sensitive detection and quantification of labeled targets.

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2 protocols using fluorescent 700 800 secondary antibodies

1

Notch Signaling Pathway Evaluation

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Penicillin, streptomycin, tetracycline, Dulbecco’s Modified Eagle’s medium, and fetal bovine serum were obtained from Invitrogen (Life Technologies, Carlsbad, CA, USA). The protein assay kit was from Bio-Rad (Hercules, CA, USA). Fibronectin and S100A4 were from Sigma-Aldrich (St. Louis, MO, USA); CD31 antibodies were from BD Biosciences (BD Biosciences, San Jose, CA, USA); antibodies against Jagged 1, and Hes 1, Hey 1, and Hes 5 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against Notch 1, N1ICD, RBP-Jκ, and iNOS were from Cell Signaling Technology (Danvers, MA, USA). The fluorescent-700/800 secondary antibodies were obtained from Invitrogen (Carlsbad, CA, USA), an antibody against CD45 was from Millipore (Billerica, MA, USA), and antibodies against F4/80, proliferating cell nuclear antigen, GFP, and rabbit anti-α-SMA were from Abcam (Cambridge, MA, USA). The GFP antibody was purchased from Rockland Immunochemicals (Limerick, PA, USA), and lipopolysaccharide and recombinant IL-4 were purchased from R&D Systems (Minneapolis, MN, USA). BrdU Labeling and Detection Kits were obtained from Roche (Indianapolis, IN, USA).
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2

Notch Signaling Pathway Activation

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Penicillin, streptomycin, DMEM, Dynabeads of sheep anti-rat IgG and FBS were obtained from Invitrogen (Life Technologies, Carlsbad, CA). The protein assay kit was from Bio-Rad (Hercules, CA). Monoclonal α-smooth muscle actin (α-SMA)-FITC antibodies were from Sigma-Aldrich (St. Louis, MO) and Hes1, Hey1 and Hes5 antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against Notch1, against the active cleaved Notch1 (N1ICD), RBP-Jκ, and iNOS were from Cell Signaling Biotechnology (Cell Signaling, Danvers, MA). The fluorescent-700/800 secondary antibodies were obtained from Invitrogen (Carlsbad, CA), while an antibody against CD45 was from Millipore (Billerica, MA) and antibodies against F4/80, PCNA, GFP, rabbit anti-α-SMA were from Abcam (Cambridge, MA). The GFP antibody was purchased from Rockland (Limerick, PA) and recombinant human TGF-β1, LPS, IL-1β, FGF-2 were purchased from R&D Systems (Minneapolis, MN). Monocyte chemotactic protein-1 (Cedarlane, Burlington, NC). The adenovirus vector N1ICD were generously provided by Dr. Liaw (Maine Medical Center Research Institute, Scarborough, ME).38 (link) Cell lysates were prepared 48–72 hours after adenovirus infection for western blot.
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