Il 17a elisa kit

Manufactured by R&D Systems

Sourced in United States

The IL-17A ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of IL-17A levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Opteia mouse elisa set, by BD (1 mentions) Cd4 cd62l t cell isolation kit, by Miltenyi Biotec (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Mog35 55 peptide, by Thermo Fisher Scientific (1 mentions) Cd11c positive isolation kit, by Miltenyi Biotec (1 mentions) Tgf β, by R&D Systems (1 mentions) Latent tgf β, by R&D Systems (1 mentions) X vivo 15 chemically defined serum free hematopoietic cell medium, by Lonza (1 mentions) Taqman assay, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by FlowJo (1 mentions)

Close spelling variants of this product

IL-17A (157 citations) ELISAs (144 citations) Mouse IL-17A ELISA kit (3 citations) Murine IL-17A ELISA kits (2 citations) Canine IL‐17/IL‐17A Quantikine ELISA Kit (2 citations) Mouse IL-17A ELISA DUO set kit (2 citations) Mouse IL-17A Quantikine ELISA Kit (2 citations)

4 protocols using il 17a elisa kit

Mouse Cytokine Profiling by ELISA

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Mouse cytokine levels in culture supernatants were determined by commercially available ELISA kits following the manufacturer’s instructions. The IL-17A ELISA kit was from R&D Systems. The IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p40, TNF-α and IFN-γ BD OptEIA-Mouse ELISA Sets were from BD Biosciences Pharmigen.

Mourglia-Ettlin G., Miles S., Velasco-De-Andrés M., Armiger-Borràs N., Cucher M., Dematteis S, & Lozano F. (2018). The ectodomains of the lymphocyte scavenger receptors CD5 and CD6 interact with tegumental antigens from Echinococcus granulosus sensu lato and protect mice against secondary cystic echinococcosis. PLoS Neglected Tropical Diseases, 12(11), e0006891.

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Cytokine Production Measurement Protocol

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Cells were harvested after indicated stimulation periods and supernatants were collected to measure cytokine production according to manufacturer’s instructions. IL-2 and IL-10 ELISA kits were obtained from eBioscience and IL-17A ELISA kit was obtained from R&D systems.

Pai C., Walsh C.M, & Fruman D.A. (2016). Context-specific function of S6K2 in helper T cell differentiation. Journal of immunology (Baltimore, Md. : 1950), 197(8), 3049-3058.

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Induction of Th17 Cells from Naive T Cells

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Splenic DCs were sorted to more than 95% purity using the CD11c Positive Isolation Kit (Miltenyi Biotec). Naive 2D2 T cells were isolated from the lymph nodes and spleens of 2D2 TCR transgenic mice using the CD4⁺CD62L⁺ T-cell isolation kit (Miltenyi Biotec). Co-culture of CD4⁺ T cells with splenic DCs has been described previously (29 (link), 30 (link)). Briefly, purified naive 2D2 CD4⁺ T cells were cultured with splenic DCs at a ratio of 5:1 in the presence of the MOG_35–55 peptide (10 µg/ml), IL-6 (50 ng/ml; Peprotech), IL-1β (10 ng/ml; Peprotech), FICZ (6-formylindolo [3,2-b] carbazole; 300 nM; Biomol) and TGF-β (1 ng/ml; R&D Systems) or latent-TGF-β (50 ng/ml). For RGD blockade experiments, cRGD peptide was added at 4 µg/ml. For the co-culture assay, co-cultured cells were maintained in X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium (Lonza). The 2D2 T cells were analyzed by flow cytometry after 3 days of culture. Cytokines in the culture supernatants were measured using an IL-17A ELISA kit (R&D, USA). The activation of TGF-β was measured with the Plasminogen Activator Inhibitor-1 Promoter Luciferase Assay (details described below).

Su P., Chen S., Zheng Y.H., Zhou H.Y., Yan C.H., Yu F., Zhang Y.G., He L., Zhang Y., Wang Y., Wu L., Wu X., Yu B., Ma L.Y., Yang Z., Wang J., Zhao G., Zhu J., Wu Z.Y, & Sun B. (2016). Novel function of Extracellular matrix protein 1 in suppressing Th17 cell development in experimental autoimmune encephalomyelitis. Journal of immunology (Baltimore, Md. : 1950), 197(4), 1054-1064.

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Cytokine Expression in Autoimmune Rats

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Mononuclear cells from spleen were isolated from F1[21-3x283-2] rats at day 7, 14, 21 after immunization with MTB in IFA (n=4/time point). At each time point two non-immunized rats matched by age and gender, were taken along as control. The non-immunized rats (n=6) were pooled for all analyses. Cells were used either unstimulated or after ex vivo restimulation with 10 ng/ml PMA and 1 µg/ml ionomycin for 6 hours. Using splenocytes, mRNA expression was measured using Taqman assays (Thermo Fisher Scientific) for il17a (assay ID Rn01757168_m1), tnf (assay ID Rn99999017_m1) and ifng (assay ID Rn00594078_m1) with gapdh (assay ID Rn01775763_g1) as housekeeping gene. Data were analyzed according to the 2^-ΔΔCT method (29 (link)). IL-17A, TNF, and IFNy protein secretion was measured by ELISAs in the culture supernatant stimulated for 24 hours (Thermo Fisher Scientific IL-17A ELISA kit #88-7170-88; R&D duoset IFNy ELISA #DY-585). Intracellular protein expression of IL-17A and IFNy (versus isotype control) by different lymphocyte subsets was assessed by FACS in splenocytes stimulated overnight (in the presence of 10 µg/ml Brefeldin for the final 4 hours). Data was recorded using a FACS Canto II and analyzed using FlowJo software.

van Tok M.N., Mandour M., Wahle J., Labadia M.E., van de Sande M.G., Nabozny G., Baeten D.L, & van Duivenvoorde L.M. (2021). Paradoxical Augmentation of Experimental Spondyloarthritis by RORC Inhibition in HLA-B27 Transgenic Rats. Frontiers in Immunology, 12, 699987.

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