C. perfringens (ATCC 13124) was revived according to standard procedures in tryptose sulfite-cycloserine agar (Oxoid) with d -cycloserine (Sigma) under anaerobic conditions at 36 ± 1 °C for 24 h.11 The bacterial concentration in the inoculum was standardized at 0.5 on the McFarland turbidity scale, equivalent to 108 CFU mL–1. An aliquot (1 mL) of this suspension was transferred to a sterile tube and the volume was adjusted to 10 mL with sodium chloride solution (0.8%, w/v) to obtain a concentration of 107 CFU mL–1. Working inoculums were prepared by transferring 200 μL aliquots of this suspension to three test tubes and adjusting the volumes to 10 mL with reinforced clostridial medium (RCM; Oxoid) to give final concentrations of 2.0 × 105 CFU mL–1.12 (link)
Tryptose sulfite cycloserine agar
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Tryptose sulfite-cycloserine agar is a solid microbiological growth medium used for the isolation and identification of Clostridium species, particularly Clostridium perfringens. It contains tryptose as a source of amino acids and other nutrients, sodium sulfite, and cycloserine, which inhibit the growth of many non-clostridial bacteria.
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Lab products found in correlation
3 protocols using tryptose sulfite cycloserine agar
1
Reviving and Standardizing C. perfringens Inoculum
2
Histological Tissue Processing Procedures
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Formaldehyde, ethanol, xylol, paraffin, hematoxylin, and eosin were from M/S Merck KGaA, Darmstadt, Germany. Equipment, such as a light microscope, thermocycler, and AlphaImager Mini Imaging System, was from Nikon (Japan), Bio-Rad (UAE), and ProteinSimple (UK), respectively. Tryptose sulfite cycloserine agar and agar agar were from Oxoid, UK, API 20A kits were from bioMérieux, Marcy l'Étoile, France, Column Extraction Kit was from Bio-Basic, Canada, and other chemicals for PCR were from Wuhan Zokeyo Co. Ltd., China. The Minitab software for Windows was from Minitab Ltd., Coventry, UK.
3
Antimicrobial Effects of ZnO and Nano-ZnO on Foodborne Pathogens
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An in vitro microbial test was performed to evaluate the antimicrobial effects of
ZnO (0, 300, 500, and 2,500 ppm) and nano-ZnO (300 and 500 ppm) mixed with
nutrient broth, following the method of Joray et al. [24 (link)]. To test the antibacterial effects against
Escherichia coli O157:H743895ATCC, Salmonella
enteritidis ATCC 13076, and Listeria monocytogenes
Scott A 19119 ATCC, 108 CFU/mL were mixed in the flasks. The
inoculated broths were shaken at 120 rpm at 37°C for 24 h, and samples
were taken at h 3, 6, 9, 12, and 24. The collected samples were serially diluted
in sterile water and incubated on plate count agar. As per Hosseindoust et al.
[12 (link)], fecal samples were collected on
days 14 and 28 and sealed for microbiological analysis. The analysis was
performed immediately after fecal collection. Lactobacillusspp. (de man, rogosa and sharp [MRS] agar + 0.200 g/L NaN3 + 0.500 g/L L-cystine
hydrochloride monohydrate), coliforms (violet red bile agar), and
Clostridium spp. (tryptose sulfite cycloserine agar, Oxoid,
Hampshire, UK) were counted. For microbial groups, anaerobic conditions were
generated using a Gaspak anaerobic system (BBL, No. 260678, Difco, Detroit, MI,
USA). CFUs were used to determine microbial populations and transformed into
log10.
ZnO (0, 300, 500, and 2,500 ppm) and nano-ZnO (300 and 500 ppm) mixed with
nutrient broth, following the method of Joray et al. [24 (link)]. To test the antibacterial effects against
Escherichia coli O157:H743895ATCC, Salmonella
enteritidis ATCC 13076, and Listeria monocytogenes
Scott A 19119 ATCC, 108 CFU/mL were mixed in the flasks. The
inoculated broths were shaken at 120 rpm at 37°C for 24 h, and samples
were taken at h 3, 6, 9, 12, and 24. The collected samples were serially diluted
in sterile water and incubated on plate count agar. As per Hosseindoust et al.
[12 (link)], fecal samples were collected on
days 14 and 28 and sealed for microbiological analysis. The analysis was
performed immediately after fecal collection. Lactobacillusspp. (de man, rogosa and sharp [MRS] agar + 0.200 g/L NaN3 + 0.500 g/L L-cystine
hydrochloride monohydrate), coliforms (violet red bile agar), and
Clostridium spp. (tryptose sulfite cycloserine agar, Oxoid,
Hampshire, UK) were counted. For microbial groups, anaerobic conditions were
generated using a Gaspak anaerobic system (BBL, No. 260678, Difco, Detroit, MI,
USA). CFUs were used to determine microbial populations and transformed into
log10.
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