Anti msh6

Manufactured by Fortis Life Sciences

Anti-MSH6 is a laboratory product used for research purposes. It is a protein that functions as a component of the DNA mismatch repair system.

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Lab products found in correlation

Anti msh2, by BD (1 mentions) Anti actin, by Thermo Fisher Scientific (1 mentions) Anti pcna pc10, by Santa Cruz Biotechnology (1 mentions) Anti pol η, by Abcam (1 mentions) Anti aid, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Anti fancd2, by Santa Cruz Biotechnology (1 mentions) Anti alpha tubulin, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti msh6

Quantifying MSH2 and MSH6 Protein Levels

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Approximately one million cells for each line under study were lysed in
200 µL RIPA buffer in presence of protease inhibitors. 25 µg of
each cell lysate was run on an 8% SDS-PAGE gel and probed with anti-MSH2 (BD
Pharmingen, 1:1000), anti-MSH6 (Bethyl laboratories, 1:1000), and
anti-beta-actin (Sigma-Aldrich, 1:2000) antibodies. Percentage estimation of
MSH2 and MSH6 levels in variant expressing lines were carried out in comparison
to WT H1 MSH2 or MSH6 levels after being normalized for protein loading using
beta-actin levels via ImageJ software.

Rath A., Mishra A., Ferreira V.D., Hu C., Omerza G., Kelly K., Hesse A., Reddi H.V., Grady J.P, & Heinen C.D. (2019). Functional Interrogation of Lynch Syndrome Associated MSH2 Missense Variants via CRISPR-Cas9 Gene Editing in Human Embryonic Stem Cells. Human mutation, 40(11), 2044-2056.

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Protein Expression Analysis in Cell Lysates

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Samples were collected and placed on ice in a lysis solution [50 mM Tris–HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1% NP-40, 10% glycerol] containing 0.5% SDS and 2 mM PMSF with a protease inhibitor cocktail (Sigma P-8340, 1:100). Cellular proteins were resolved on a 12.5% SDS–PAGE gel. The membrane was incubated for 1 h at room temperature in 5% skim milk in PBS with 0.05% Tween-20 (PBST), and the membrane was probed with anti-PCNA PC10 (Ref # sc56; Santa Cruz), anti-alpha tubulin (Ref# MA1-80017; Thermo Fisher Scientific), anti-actin (Ref #MA1-744; Thermo Fisher Scientific), anti-Vinculin (clone 7F9, Ref# 14-9777-80; eBioscience), anti-AID (Ref #14-959-82; Thermo Fisher Scientific), anti-polη (Ref# ab17725; Abcam), anti-FancD2 (Ref# sc20022; Santa Cruz), anti-USP1 (Ref # ab108104Ref; Abcam), anti-Msh2 (Ref #A300-451A; Bethyl), and anti-Msh6 (Ref # A300-022A; Bethyl) antibodies. Immunoreactivity was detected using a horseradish peroxidase-conjugated secondary antibody.

Lerner L.K., Bonte D., Le Guillou M., Mohammad M.M., Kasraian Z., Sarasin A., Despras E, & Aoufouchi S. (2022). Expression of Constitutive Fusion of Ubiquitin to PCNA Restores the Level of Immunoglobulin A/T Mutations During Somatic Hypermutation in the Ramos Cell Line. Frontiers in Immunology, 13, 871766.

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