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20x 1.0 na

Manufactured by Zeiss
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The Zeiss 20x (1.0 NA) is a high-performance microscope objective lens. It provides a 20x magnification and a numerical aperture of 1.0, which enables the collection of a large amount of light and high-resolution imaging.

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Lab products found in correlation

Agarose sfr, by Avantor (2 mentions) Methylcellulose, by Merck Group (2 mentions) Pm100d, by Thorlabs (1 mentions) Fluar 5x 0.25na, by Zeiss (1 mentions)

Close spelling variants of this product

W-Plan-Apo/1.0 NA 20x water immersion objective

3 protocols using 20x 1.0 na

1

In vivo Zebrafish Imaging Protocol

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For in vivo imaging experiments, zebrafish were anesthetized in approximately 0.2 mM MS-222 Tricaine-S (Western Chemical, Inc., Ferndale, WA) diluted in E3 medium, then placed in 60 mm Petri dishes, mounted for a dorsal or sagittal view on agarose pads (3%), and embedded in 1% low-melt agarose (Agarose SFR; AMRESCO, Solon, OH). For short interval time-lapses, fish remained on the microscope for the duration of the time observed. For 2 and 3 dpf time points in Fig. 2A only, fish were removed from the microscope, taken out of agarose and returned to Petri dishes at 28 °C in between time points. During this 24 h time period the brain tissue grows significantly and can lead to slight changes in cell position and depth. Images and movies of live-mount zebrafish were taken with a Zeiss 20x (1.0 NA) water immersion objective with zoom ranging from 1.0 to 2.5. For time-lapse imaging, the time interval between captured Z-stacks was 12, 30, or 32 min. Brightfield images in Fig. 1A were taken on a Zeiss Discovery V8 stereo microscope, with fish mounted in 3% methyl cellulose (Sigma, St. Louis).
Supplementary data related to this article can be found online at https://doi.org/10.1016/j.ydbio.2019.05.006
Brockway N.L., Cook Z.T., O’Gallagher M.J., Tobias Z.J., Gedi M., Carey K.M., Unni V.K., Albert Pan Y., Metz M.R, & Weissman T.A. (2019). Multicolor lineage tracing using in vivo time-lapse imaging reveals coordinated death of clonally related cells in the developing vertebrate brain. Developmental biology, 453(2), 130-140.
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2

Calibrating Fluorescence Imaging Power

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To ensure the reliability of our fluorescence imaging measurements, we periodically tested the LED power output using a visible light sensor (Thorlabs, S120C) and power meter (Thorlabs, PM100D). For consistent power measurements across time, we centered the illumination profile on the center of the sensor and by directly focusing on the surface using the microscope’s software (SlideBook 6), we ensured that the sensor surface was in the focal plane of the microscope. We then used custom MATLAB scripts to create a calibration curve between the software’s arbitrary power scale and power (mW) or irradiance (mW/cm2). For all one-photon imaging experiments we acquired data with LED power set to 30–40 in SlideBook, which corresponded to total power (at given SlideBook power) of blue excitation light at the focal plane of 3.52 (30) and 4.28 (40) mW with the Zeiss Fluar 5x/0.25NA, 2.3 (30) and 2.78 (40) mW with the Zeiss 5x/0.16NA, 5.47 (30) and 6.61 (40) mW with the Zeiss 20X/1.0NA, and 3.71 (30) and 4.49 (40) mW with the Olympus 20X/0.45NA.
Ahanonu B., Crowther A., Kania A., Casillas M.R, & Basbaum A. (2023). Long-term optical imaging of the spinal cord in awake, behaving animals. bioRxiv.
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3

In vivo Zebrafish Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
For in vivo imaging experiments, zebrafish were anesthetized in approximately 0.2 mM MS-222 Tricaine-S (Western Chemical, Inc., Ferndale, WA) diluted in E3 medium, then placed in 60 mm Petri dishes, mounted for a dorsal or sagittal view on agarose pads (3%), and embedded in 1% low-melt agarose (Agarose SFR; AMRESCO, Solon, OH). For short interval time-lapses, fish remained on the microscope for the duration of the time observed. For 2 and 3 dpf time points in Fig. 2A only, fish were removed from the microscope, taken out of agarose and returned to Petri dishes at 28 °C in between time points. During this 24 h time period the brain tissue grows significantly and can lead to slight changes in cell position and depth. Images and movies of live-mount zebrafish were taken with a Zeiss 20x (1.0 NA) water immersion objective with zoom ranging from 1.0 to 2.5. For time-lapse imaging, the time interval between captured Z-stacks was 12, 30, or 32 min. Brightfield images in Fig. 1A were taken on a Zeiss Discovery V8 stereo microscope, with fish mounted in 3% methyl cellulose (Sigma, St. Louis).
Supplementary data related to this article can be found online at https://doi.org/10.1016/j.ydbio.2019.05.006
Brockway N.L., Cook Z.T., O’Gallagher M.J., Tobias Z.J., Gedi M., Carey K.M., Unni V.K., Albert Pan Y., Metz M.R, & Weissman T.A. (2019). Multicolor lineage tracing using in vivo time-lapse imaging reveals coordinated death of clonally related cells in the developing vertebrate brain. Developmental biology, 453(2), 130-140.
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