All procedures were carried out at room temperature unless otherwise stated. Samples were agitated only during antibody incubation and washes. Particular attention was paid to maintaining an RNase‐free environment. To help avoid contamination, solutions prepared according to Jezzini et al. (2005 (link)) were made in small batches in disposable sterile 50 ml plastic centrifuge tubes (Corning Incorporated, NY, USA; Cat. No. 430921). The specimens were incubated in disposable sterile 24‐well cell culture plates (Corning Incorporated, NY, USA; Cat. No. 3524).
50 ml plastic centrifuge tubes
Manufactured by Corning
Sourced in United States
The 50 mL plastic centrifuge tubes are laboratory equipment designed for use in centrifugation processes. These tubes provide a standardized container for holding samples during high-speed spinning, enabling the separation of particles, cells, or other components within the sample.
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3 protocols using 50 ml plastic centrifuge tubes
1
Preserving RNA Integrity in Experimental Protocols
2
Maintaining RNase-free Environment for Antibody Incubation
3
Uranium Adsorption on Hydroxyapatite
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All adsorption experiments were conducted in duplicates, including blanks and calibration controls. Briefly, 20 mg of HA was weighed into 50 mL plastic centrifuge tubes (Corning, Corning, NY, USA) with 30 mL U solution, and the pH of the suspension was adjusted to 3.0. The tubes were then shaken for 6 h to achieve equilibrium. Then, the tubes were centrifuged, and the supernatants were filtered through a 0.45 μm membrane (Whatman, Little Chalfont, Buckinghamshire, UK) for analysis of U concentration with an ICP-MS (Varian Inc., Palo Alto, CA, USA). The pH at the beginning and end of adsorption experiment was measured by a pH meter (Oakton, Vernon Hills, IL, USA).
U adsorption on the HA was calculated from the difference in concentrations before and after the adsorption. MS-Excel and OriginPro 8.0 (OriginLab, Wellesley Hills, MA, USA) were used for data processing.
U adsorption on the HA was calculated from the difference in concentrations before and after the adsorption. MS-Excel and OriginPro 8.0 (OriginLab, Wellesley Hills, MA, USA) were used for data processing.
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