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2 protocols using rabbit anti stat1 10144 2 ap

1

Immunoblotting and Flow Cytometry Antibodies

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The following antibodies were used in immunoblotting: rabbit anti-B7-H3 (#14058), rabbit anti-PD-L1 (#13684), rabbit anti-CD86 (#91882), rabbit anti-CD47 (#63000), rabbit anti-Phospho-Stat1 (Tyr701) (#9167), rabbit anti-Phospho-Stat1 (Ser727) (#8826) and rabbit anti-Phospho-Stat3 (Tyr705) (#9145) were purchased from Cell Signaling Technology (Boston, USA). Rabbit anti-Stat1 (10144-2-AP), rabbit anti-Stat3 (10253-2-AP), rabbit anti-HuR (11910-1-AP), rabbit anti-HMGCR (13533-1-AP) and rabbit anti-TTP (12737-1-AP) were from Proteintech (Chicago, USA). Mouse anti-Galectin-9 (ab153673, abcam, Cambridge, UK), rabbit anti-GAPDH (db106, Diagbio, Hangzhou, China), goat anti-mouse PD-L1 (AF1019, R&D systems, Minnesota, USA). The antibodies for flow cytometry: FITC Rat IgG2b κ isotype control (#400605), FITC Rat IgG2a κ isotype control (#400506), PE Rat IgG2a λ isotype control (#400635), PE anti-mouse PD-L1 (#124307), FITC anti-mouse CD3 (#100204), FITC anti-mouse CD8a (#100705) and PE anti-mouse CD45 (#103106) were form Biolegend (Chicago, USA).
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2

Antibody Sources for Protein Detection

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Mouse anti-RUNX1(A-2) (sc-365644) antibodies were purchased from Santa Cruz, Dallas, TX, USA; rabbit anti-IRF3 (11312-AP) and rabbit anti-STAT1 (10144–2-AP) were purchased from Proteintech, Rosemont, IL, USA; rabbit anti-P-IRF3 (Ser396) (4D4G) antibodies and rabbit anti-P-STAT1 (Tyr701) (D4A7) antibodies were purchased from Cell Signaling, Danvers, MA, USA; mouse anti-GAPDH antibody (AF0006) was purchased from Beyotime, Shanghai, China; mouse mAbs to viral proteins NP and M1 of IAV were obtained from Dr. Jiyong Zhou [35 (link)].
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