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2 protocols using dimethylformamide

1

Peptide Synthesis and Cell Viability Assay

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Fmoc-Phe-OH, Fmoc-Pro-OH, Fmoc-His(Trt)-OH, Fmoc-Cys(Trt)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Ala-OH, Rink amide-AM resin, 1-hydroxybenzotriazole, benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate, and N-methylmorpholine were obtained from GL Biochem (Shanghai, China). Dimethylformamide, methanol, dichloromethane, and n-hexane were obtained from Beijing Chemical Factory (Beijing, China). Pluronic® F-127 and DMSO were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dess–Martin periodinane was provided by Aladdin (Shanghai, China). DMEM medium was purchased from Thermo Fisher Scientific (Shanghai, China). Fetal bovine serum was purchased from Kang Yuan Biology (Tianjin, China). Trypsin and Hoechst 33258 nuclear dye were obtained from Solarbio (Beijing, China). Thiazolyl blue tetrazolium bromide (MTT) was purchased from Genview (Beijing, China). WST-1 kit and Lysotraker Green procured from Beyotime (Shanghai, China). Annexin V-FITC/PI apoptosis detection kit was purchased from BestBio (Shanghai, China).
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2

Synthesis of PAN-based MOF Materials

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PAN Mw = 70,000) was purchased from Toray Resin Company (Tokyo, Japan). Methanol, 2, 5-dihydroxyterephthalic acid, zinc acetate, dimethylformamide (DMF), and hydrochloric acid were obtained from Beijing Chemical Works (Beijing, China) and used without further purification.
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