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5 protocols using milli q rg unit

1

Quantification of Polyphosphates using Ion Chromatography

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Sodium hexametaphosphate (65-70% P2O5 basis) was supplied by Merck KGaA (Darmstadt, Germany), and carbonate-free sodium hydroxide (50%, w/w) was purchased from Fisher Scientific Italia (Rodano, Milan, Italy). All solutions were prepared by using ultrapure water with a specific resistance of 18.2 MΩ-cm, produced by a Milli-Q RG unit, Millipore (Bedford, MA, USA). The solutions used as mobile phase, composed of different concentrations of sodium hydroxide, were filtered using 0.45-μm membranes and degassed with nitrogen. The standard solutions of PPs were prepared from the 1000 mg L-1 stock solution, diluting with ultrapure water to obtain the following concentrations: 12.5, 25, 50, 100 and 200 mg L-1.
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2

Quantitative Analysis of LSD in Oral Fluid

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The LSD, OH-LSD and LSD-d 3 (internal standard, IS) standards were purchased from Cerilliant (Round Rock, TX, USA); isopropanol, dichloromethane, methanol, acetonitrile and formic acid (98-100%) from Merck (Darmstadt, Germany); sodium tetraborate from LS Chemicals (Ribeirao Preto, SP, Brazil). Ultrapure deionized water was supplied by a Milli-Q RG unit from Millipore (Billerica, MA, USA). Helix pomatia β-glucuronidase (Type HP-2, ≥ 100.000 units/mL) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All solvents employed in the extraction were of HPLC grade. Quantisal™ oral fluid collection devices and its extraction buffer (phosphate buffer solution: PBS, 100 mmol/L) were purchased from Immunalysis (Pomona, CA, USA).
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3

Quantitative Analysis of N-Ethylnorpentylone

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N-Ethylnorpentylone reference material was purchased from Cayman Chemical (Ann Arbor, MI, USA) and MDMA-d5, used as internal standard (IS), was purchased from Cerilliant (Round Rock, TX, USA). Methanol and formic acid (98-100%) were purchased from Scharlau (Barcelona, Spain). Ammonium formate and tert-butyl methyl ether (MTBE) were purchased from Sigma-Aldrich (St Louis, MO, USA) and sodium tetraborate from LS Chemicals (Ribeirao Preto, SP, Brazil). Ultrapure deionized water was purified by a Milli-Q RG unit from Millipore (Billerica, MA, USA). All solvents employed in the sample extractions were HPLC grade. N-Ethylnorpentylone, pentylone and methylone used for monoamine transporter assays were obtained from Cayman Chemical. [3H]Norepinephrine (60 Ci/mmol) and [3H]methyl-4-phenylpyridinium ([3H]MPP+; 80 Ci/mmol) were purchased from American Radiolabeled Chemicals (St. Louis, MO, USA) while [3H]dopamine (28 Ci/mmol) and [3H]5-HT (38 Ci/mmol) were purchased from Perkin Elmer (Billerica, MA, USA). All other reagents used for monoamine transporter assays were obtained from Sigma-Aldrich unless otherwise indicated.
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4

Purification and Synthesis of Peptides

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The monomer of ortho-aminophenol, oAP, (Fluka, purity 99%, FW = 109.13 g/mol) was purified, before being used, by recrystallization in ethyl acetate (Sigma Aldrich, purity 99%, d = 0.9 g/mL, bp = 76.5 °C) and with activated carbon, as suggested by Ortega [47] . Monomer oAP solutions were prepared in supporting electrolyte just before their use. The procedures for the synthesis of unprotected VGGVG and Boc-VGGVG-OEt pentapeptides and for the pentadecapeptide (VGGVG) 3 are reported in details in previous studies [34] [35] [36] 48] . Pure water supplied by Milli-Q RG unit from Millipore (Bedford, MA, USA) was used throughout. All other chemicals were of analytical grade and were used without further purification.
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5

Certified Standards for Ion Chromatography

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Certified reference material (CRM) of NO3 -and NO2 -standard solutions for ion chromatography (1000 mg/L) were purchased from Sigma-Aldrich (Stenheim, Germany). Ultrapure water supplied by a Milli-Q RG unit from Millipore (Bedford, MA, USA) with a specific resistance of 18.2 MΩ cm -1 , was used to prepare all standard solutions, mobile phases and for sample preparation. Working solutions were prepared by dilution CMR in ultrapure Milli-Q water to get the required concentrations.
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