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2 protocols using afzelin

1

Phytochemical Analysis and Antioxidant Evaluation

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Na2CO3, Al(NO3)3, formic acid, NaNO2, HCl, ethanol, Folin–Ciocalteu phenol reagent, and Trolox were obtained from Chengdu Kelong Chemical Reagent Works (Chengdu, China). 1,1-Diphenyl-2-picrylhydrazyl (DPPH·, >99.7%), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS·+, >99.7%), and chromatographic-grade methanol and acetonitrile were purchased from American Sigma (St. Louis, MO, USA). Standard compounds including (+)-catechin, (−)-epicatechin, rutin, afzelin, astragaline, kaempferol-3-O-rutinoside, kaempferol, quercetin, isoquercitrin, pinocembrin, pinocembrin 7-O-glucoside, thonningianin A, gallic acid, and pinocembrin 7-O-(3″-O-galloy-4″,6″-hexahydroxydiphenoyl)-glucoside were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Alcohol dehydrogenase (265 units/mg) from saccharomyces cerevisiae was purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA).
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2

Phytochemical Characterization and Antioxidant Assays

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Folin-Ciocalteu phenol reagent, Na 2 CO 3 , formic acid, Al(NO 3 ) 3 , NaNO 2 , HCl, H 2 SO 4 , methanol, ethanol, and Trolox were obtained from Chengdu Kelong Chemical Reagent Works (Chengdu, China). 1,1-Diphenyl-2-picrylhydrazyl (DPPH, >99.7%), 2,2 ′ -azino-bis(3ethylbenzo thiazoline-6-sulfonic acid) diammonium salt (ABTS+, >99.7%), Oil red O (ORO), p-ntrophenyl laurate (pNP laurate), and chromatographic-grade methanol and acetonitrile were purchased from American Sigma (St. Louis, MO, USA). Standard compounds, including rutin, afzelin, astragalin, kaempferol-3-O-rutinoside, isoquercitrin, (+)-catechin, procyanidin B2, cyanidin-3-O-glucoside, 1,2,3,6-tetragalloylglucose, simvastatin, orlistat, glycocholic acid, cholic acid, taurocholic acid, porcine pancreatic lipase, and tris-HCl buffer solution, were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Standard compounds, including procyanidin B1, procyanidin B4, and procyanidin C1, were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China).
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