3-Trimethylsilylpropionic acid (TSP) and Streptozotocin (STZ) were obtained from Sigma-Aldrich (St. Louis, USA). Deuterium oxide (D2O, 99.9%), deuterated methanol (CD3OD, 99.9%), potassium dihydrogren phosphate, deuterated sodium hydroxide and sodium azide were purchased from Merck (Darmstadt, Germany). The normal rat chow feed was purchased from Specialty feeds (Glen Forrest, Australia).
Deuterated methanol cd3od
Manufactured by Merck Group
Sourced in Germany, United States
Deuterated methanol (CD3OD) is a stable isotope-labeled chemical compound used in various analytical and research applications. It is a colorless, volatile liquid with a chemical formula of CD3OD. Deuterated methanol is primarily used as a solvent and reagent in nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and other analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Deuterated sodium hydroxide, by Merck Group (2 mentions)
3 trimethylsilylpropionic acid tsp, by Merck Group (2 mentions)
Secologanoside, by Sichuan Weikeqi Biological Technology (2 mentions)
Deuterium oxide d2o, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Trifluoroacetic acid, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Avantor (2 mentions)
Normal rat chow feed, by Specialty Feeds (1 mentions)
Deuterium oxide, by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Dextromethorphan hydrobromide dxm, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by Merck Group (1 mentions)
Potassium dihydrogen phosphate, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Lc ms grade methanol, by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Deuterated chloroform cdcl3, by Merck Group (1 mentions)
Folin ciocalteu phenol reagent, by Merck Group (1 mentions)
Lichroprep rp 18 gel, by Merck Group (1 mentions)
10 protocols using deuterated methanol cd3od
1
Metabolic profiling of rat diabetes
2
NMR and LCMS Analysis of Rat Chow
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All rats consumed the normal rat chow from Specialty Feeds (Glen Forrest, Australia). The phosphate buffer saline (PBS), lipopolysaccharide (LPS) derived from Escherichia coli 026: B6, and dextromethorphan hydrobromide (DXM) were obtained from Sigma Aldrich (St. Louis, United States). The NMR analysis used solvents in deuterated form, including deuterium oxide (D2O, 99.9%), deuterated methanol (CD3OD, 99.9%), deuterated sodium hydroxide and potassium dihydrogen phosphate were purchased from Merck (Darmstadt, Germany), and 3-Trimethylsilyl propionic acid (TSP) was purchased from Sigma Aldrich (St. Louis, United States). The LCMS analysis acquired LC-MS grade methanol, acetonitrile, water, and 0.1% formic acid from Merck (Darmstadt, Germany).
3
Profiling and Authenticating Lonicerae Samples
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A total of 100 batches of LJF and LF samples were collected from all over China, including 77 batches of cultivated Lonicerae japonicae flos (cLJF) with different processing methods and different origins, 10 batches of wild Lonicerae japonicae flos (wLJF), and 13 batches of Lonicerae flos (LF). The detailed sample information is shown in Table 3 . All the samples were authenticated by Chief Pharmacist Ji Zhang, who is the former Director of the Traditional Chinese Medicine Herbarium at the China National Institute for Food and Drug Control. Voucher samples were preserved and stored in the cold sample room of Shenzhen Institute for Drug Control.
Deuterated methanol (CD3OD, purity ≥ 99.8%) containing 0.05% (v/v) TMS was purchased from Merck. Secologanoside (purity 99.28%) analytical standard was acquired from Sichuan Weikeqi Biological Technology Co., Ltd. Secoxyloganin (Chengdu, China) (purity 98.93%) analytical standard was acquired from Shanghai Hongyong Biological Technology Co., Ltd. Macranthoidin A (Shanghai, China) (purity 100.0%) was acquired from Standard Technology Co., Ltd. (Shanghai, China). Macranthoidin B (purity 96.1%), Dipsacoside B (purity 96.8%) and Sweroside (purity 98.3%) were purchased from the National Institute for Food and Drug Control (Beijing, China). All of the other chemicals used in this study were of analytical grade.
Deuterated methanol (CD3OD, purity ≥ 99.8%) containing 0.05% (v/v) TMS was purchased from Merck. Secologanoside (purity 99.28%) analytical standard was acquired from Sichuan Weikeqi Biological Technology Co., Ltd. Secoxyloganin (Chengdu, China) (purity 98.93%) analytical standard was acquired from Shanghai Hongyong Biological Technology Co., Ltd. Macranthoidin A (Shanghai, China) (purity 100.0%) was acquired from Standard Technology Co., Ltd. (Shanghai, China). Macranthoidin B (purity 96.1%), Dipsacoside B (purity 96.8%) and Sweroside (purity 98.3%) were purchased from the National Institute for Food and Drug Control (Beijing, China). All of the other chemicals used in this study were of analytical grade.
4
Chromatographic Separation and Identification
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The solvents, dichloromethane, ethyl acetate, acetonitrile, ethanol and methanol were of HPLC grade, purchased from Fisher Chemical (Fisher Scientific, Loughborough, Leics, UK). Silica gel 0.040–0.063 mm, TLC (20 × 20 cm) of normal and reverse phase, deuterated methanol (CD3OD) and deuterated chloroform (CDCl3) were purchased from Merck KGaA (Darmstadt, Germany); olive oil was extra virgin oil “ALTIS” (ELAIS).
5
Profiling and Authenticating Lonicerae Samples
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A total of 100 batches of LJF and LF samples were collected from all over China, including 77 batches of cultivated Lonicerae japonicae flos (cLJF) with different processing methods and different origins, 10 batches of wild Lonicerae japonicae flos (wLJF), and 13 batches of Lonicerae flos (LF). The detailed sample information is shown in Table 3 . All the samples were authenticated by Chief Pharmacist Ji Zhang, who is the former Director of the Traditional Chinese Medicine Herbarium at the China National Institute for Food and Drug Control. Voucher samples were preserved and stored in the cold sample room of Shenzhen Institute for Drug Control.
Deuterated methanol (CD3OD, purity ≥ 99.8%) containing 0.05% (v/v) TMS was purchased from Merck. Secologanoside (purity 99.28%) analytical standard was acquired from Sichuan Weikeqi Biological Technology Co., Ltd. Secoxyloganin (Chengdu, China) (purity 98.93%) analytical standard was acquired from Shanghai Hongyong Biological Technology Co., Ltd. Macranthoidin A (Shanghai, China) (purity 100.0%) was acquired from Standard Technology Co., Ltd. (Shanghai, China). Macranthoidin B (purity 96.1%), Dipsacoside B (purity 96.8%) and Sweroside (purity 98.3%) were purchased from the National Institute for Food and Drug Control (Beijing, China). All of the other chemicals used in this study were of analytical grade.
Deuterated methanol (CD3OD, purity ≥ 99.8%) containing 0.05% (v/v) TMS was purchased from Merck. Secologanoside (purity 99.28%) analytical standard was acquired from Sichuan Weikeqi Biological Technology Co., Ltd. Secoxyloganin (Chengdu, China) (purity 98.93%) analytical standard was acquired from Shanghai Hongyong Biological Technology Co., Ltd. Macranthoidin A (Shanghai, China) (purity 100.0%) was acquired from Standard Technology Co., Ltd. (Shanghai, China). Macranthoidin B (purity 96.1%), Dipsacoside B (purity 96.8%) and Sweroside (purity 98.3%) were purchased from the National Institute for Food and Drug Control (Beijing, China). All of the other chemicals used in this study were of analytical grade.
6
Antioxidant Compounds Isolation and Purification
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2,2-Diphenyl-1-picrylhydrazyl (DPPH), Folin-Ciocalteu phenol reagent, trifluoroacetic acid (TFA), sulfuric acid, sodium carbonate, vanillin, gallic acid, l -ascorbic acid, (+)-catechin, deuterium oxide (D2O), and deuterated methanol (CD3OD) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The Purospher STAR RP-18e column (4.0 mm i.d. × 250 mm, 5 μm) was purchased from Merck (Darmstadt, Germany). Methanol and acetonitrile were LiChrosolv and were purchased from Merck. Diaion HP-20 gel (Mitsubishi Chemical Industry, Tokyo, Japan), Sephadex LH-20 gel (GE Healthcare Biosciences, Uppsala, Sweden), LiChroprep RP-18 gel (40–63 μm, Merck), MCI CHP20P gel (Supelco, Bellefonte, PA, USA) and an Oasis HLB cartridge (12 mL, 500 mg, Waters, Milford, MA, USA) were used for the purification and isolation processes. Polyvinylidene difluoride (PVDF) 0.45-μm 13 mm syringe filters was purchased from IT’S Science Corporation (Taipei, Taiwan).
7
Synthesis and Characterization of Polymeric Biomaterials
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Dimethyl acrylamide (DMAm, 99 %; Sigma Aldrich 274135), Acrylic Acid (AA, 99 %; Sigma Aldrich 147230), (Methacryloyloxy)ethyl] trimethylammonium chloride (QDMAEMA, 75 % in H2O; Sigma Aldrich 408107), (Methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (DMAPS, 95 %; Sigma Aldrich 537284), 4,4′-azobis(4-cyanovaleric acid) (ACVA, 99%; Sigma Aldrich 11590), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, 98%; Sigma Aldrich 341006), deuterated methanol (CD3OD, 99.8%; Sigma Aldrich 151947-10G-GL) and deuterium oxide (D2O, 99.9%; Sigma Aldrich 151882) were purchased from Sigma Aldrich (UK). DAAm (99%) was purchased from Alfa Aesar (UK; A15940.30). 4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acyl hydrazide (BODIPY FL, 99 %) was purchased from ThermoFisher Scientific (UK; D2371). 4-((((2-Carboxyethyl)thio)carbonothioyl)thio)−4-cyanopentanoic acid (BM1433, 95%) was purchased from Boron Molecular (USA; BM1433).
For protoplast preparation, cellulase “Onozuka RS” was purchased from Melford biolaboratories (Duchefa; C8003.0005) and pectolyase from Sigma Aldrich (P3026).
For protoplast preparation, cellulase “Onozuka RS” was purchased from Melford biolaboratories (Duchefa; C8003.0005) and pectolyase from Sigma Aldrich (P3026).
8
Analytical Solvent Extraction and Chromatography
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Analytical-grade solvents, including n-hexane, chloroform, and methanol, were used for the extraction and isolation procedures. The MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), HPLC-grade methanol, and deuterated methanol (CD3OD) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Cerium sulphate, used in the study, was also obtained from Sigma-Aldrich. HPLC-grade water with a resistivity of 18 MΩ was prepared using a Milli-Q50 purification system from Millipore Corp. (Bedford, MA, USA). The Sephadex LH-20 resin employed in the chromatography procedure was purchased from Supelco (Merck KGaA, Darmstadt, Germany). Furthermore, dimethyl sulfoxide (DMSO), used for solubilizing the extract and molecules during bioassays, was obtained from VWR Chemicals (VWR International Srl, Milano, Italy).
9
Analytical Reagents and Solvents Purification
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All chemicals and reagents used, including the solvents, were of analytical grade. The solvents were distilled before use. Acetone (Ref.: 20066.330), methanol (MeOH, Ref.: 20903.368), sulfuric acid (H2SO4, Ref.: 470302.872), dichlorometane (DCM, Ref.: 23367.321), and dimethyl sulfoxide (DMSO, Ref.: 23486.322) were from VWR Chemicals). Other materials were hexane (Hex, Fisher Scientific, Ref.: H/0355/21), ethyl acetate (EtOAc; LabChem, Ref.: MP013-9025), trifluoroacetic acid (Sigma-Aldrich, Ref.: 8082600101), sand pure 40–100 mesh (Acrōs organics, Ref.: 370942500); MeOH HPLC grade (Fisher Scientific, Ref.: M/4056/17); ACN HPLC grade (Honeywell, Ref.: 608-001-00-3), K2Cr2O7 (Labonal S.A., Ref.: 2635), and bidistillated water. Furthermore, filter paper (Whatman No. 5 paper, Inc., Clifton, NJ, USA), deuterated-chloroform 99.8 + 0.03% TMS (CDCl3, Deutero.de-Rotoquímica, Ref.: 00405), and deuterated methanol (CD3OD, Sigma Aldrich, Ref.: 1.06028) were used.
10
Standardized Compound Quantification Protocol
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Standards (gallic acid, lycorine, galanthamine, tyramine, tyrosine, α-glucose, β-glucose, sucrose, alanine, valine) are purchased by Sigma Aldrich (Sigma-Aldrich, St. Quentin Fallavier, France). All solvents used are analytical profile grade, deuterated methanol (CD3OD), 3-(trimethylsilyl)-1-propanesulfonic acid sodium salt (DSS sodium salt), sodium deuteroxide solution (NaOD) 40 wt.% in deuterium oxide (D2O), 99.5 atom % D, ammonium hydroxide solution (NH4OH) containing ≥25% NH3 basis, were purchased from Sigma Aldrich (Sigma-Aldrich, St. Quentin Fallavier, France). All chemicals for in vitro culture are listed in the article Saliba et al. [29 (link)]. SCX cation exchanger column are purchased by Interchim® (Interchim, Montluçon, France).
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