Mouse retina cryosections (10 μm) were fixed in 4% paraformaldehyde and blocked with 10% normal goat serum. The sections were rinsed with PBS, permeabilized with 0.1% Triton X-100, and incubated over night with anti-p38 antibody (rabbit polyclonal, ab7952, Abcam; Cambridge, MA). The slides were rinsed with PBS, and incubated with the anti-rabbit-FITC conjugated secondary antibody (NL006; R&D systems; Minneapolis, MN) for 1 hour [26 (link)]. After rinsing the slides with PBS, the sections were mounted with DAPI-containing mounting media (Vector Laboratories; Burlingame, CA), and imaged with an Olympus BX-UCB fluorescent microscope (20X magnification). The fluorescence intensity was quantified by using ImageJ software (version 1.44; NIH). Cryosections processed under similar conditions, except being incubated with the primary antibody, served as controls.
Bx ucb fluorescent microscope
Manufactured by Olympus
Sourced in Canada
The BX-UCB fluorescent microscope is a laboratory equipment designed for fluorescence microscopy. It is capable of capturing high-quality images of fluorescently-labeled samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bx ucb fluorescent microscope
1
Immunohistochemistry of p38 in Mouse Retina
2
Immunohistochemistry of p38 in Mouse Retina
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse retina cryosections (10 μm) were fixed in 4% paraformaldehyde and blocked with 10% normal goat serum. The sections were rinsed with PBS, permeabilized with 0.1% Triton X-100, and incubated over night with anti-p38 antibody (rabbit polyclonal, ab7952, Abcam; Cambridge, MA). The slides were rinsed with PBS, and incubated with the anti-rabbit-FITC conjugated secondary antibody (NL006; R&D systems; Minneapolis, MN) for 1 hour [26 (link)]. After rinsing the slides with PBS, the sections were mounted with DAPI-containing mounting media (Vector Laboratories; Burlingame, CA), and imaged with an Olympus BX-UCB fluorescent microscope (20X magnification). The fluorescence intensity was quantified by using ImageJ software (version 1.44; NIH). Cryosections processed under similar conditions, except being incubated with the primary antibody, served as controls.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!