Hematoxylin and eosin (H&E) staining was performed on 5–10-μm-thick paraffin sections, as described previously [42 ], and images were taken using an Olympus microscope (Olympus, DP70). Immunohistochemical staining was performed using paraffin-embedded tissue sections, as described previously [42 ,43 (link),45 (link),46 (link)]. Primary and secondary antibodies were used in accordance with the manufacturer's instructions. Primary antibodies including guinea pig anti-GFP (1:200) (Frontier Institute), goat anti-tdTomato (1:150) (SICGEN), rabbit anti-RFP (mouse) (1:250) (Rockland), rabbit anti-Ki67 (1:100) (Abcam), and rabbit anti-perilipin (1:100) (Santa Cruz) antibodies were used. Secondary antibodies and DAPI (Molecular Probes) were used at 1:250 and 1:500 dilutions, respectively. All images were taken using a confocal microscope (Leica TCS-SP5) and the images were analyzed using the ImageJ software.
Rabbit anti perilipin
Manufactured by Santa Cruz Biotechnology
Rabbit anti-perilipin is a primary antibody that specifically binds to the perilipin protein. Perilipin is a lipid droplet-associated protein that plays a crucial role in the regulation of lipolysis. This antibody can be used for the detection and analysis of perilipin in various biological samples using techniques such as Western blotting, immunohistochemistry, or immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Rabbit anti ki67, by Abcam (1 mentions)
Tcs sp5, by Leica (1 mentions)
Rabbit anti cleaved caspase 3 asp175 antibody, by Cell Signaling Technology (1 mentions)
Alexa fluor 568 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal anti ki 67, by BD (1 mentions)
Alexa fluor 488 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
2 protocols using rabbit anti perilipin
1
Histological and Immunohistochemical Analysis
2
Immunofluorescence Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue sections or cultured cells were fixed in 4% PFA, quenched with glycine (100 mM glycine, 0.2% Triton X-100, and 0.1% sodium azide in PBS), and blocked in PBS containing 2% BSA, 5% goat serum, and 0.2% Triton X-100 at room temperature for 45 min. Tissues or cells were then incubated with the primary antibodies diluted in the same blocking solution at 4°C overnight, and finally visualized with fluorophore-conjugated secondary antibodies (Alexa Fluor 568 goat anti–mouse IgG, A21124 Life Technologies, and Alexa Fluor 488 goat anti–rabbit IgG H&L, ab150077). The primary antibodies used were as follows: mouse monoclonal anti-Ki67 (BD), rabbit anti-cleaved caspase-3 (Asp175) antibody (Cell Signaling Technology), and rabbit anti-Perilipin (Santa Cruz Biotechnology, Inc.). Nuclei were counterstained with DAPI that was mixed with secondary antibodies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!