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Sodium assay kit

Manufactured by Nanjing Jiancheng
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The sodium assay kit is a laboratory tool used to measure the concentration of sodium ions in a variety of samples. It provides a quantitative analysis of sodium levels through a colorimetric detection method. The kit contains the necessary reagents and solutions to perform the assay.

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Lab products found in correlation

Spectramax m5 multifunctional microplate reader, by Molecular Devices (1 mentions) E el 0070c, by Elabscience (1 mentions) Potassium assay kit, by Nanjing Jiancheng (1 mentions) Agilent 7900, by Agilent Technologies (1 mentions) Bp 2010a, by Softron (1 mentions)

3 protocols using sodium assay kit

1

Urine Collection and Analysis in Mice

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For collecting urine, all experimental mice were housed individually in metabolic cages for 24 h with drinking water, without food. The mice were allowed to recover for 24 h before performing other operations. The urine output and water consumption were also measured. The urinary albumin (ml025061-j2; MIBio, Shanghai, China) and creatinine (ml026283-j2; MIBio, Shanghai, China) were detected by ELISA according to the manufacturer’s instructions. After fasting for 12 h, serum was collected. The Na levels in the urine and serum were measured by sodium assay kit (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China) using a SpectraMax M5 Multifunctional Microplate Reader (Molecular Devices, United States).
Zou M., Chen Y., Zheng Z., Sheng S., Jia Y., Wang X., Ren S., Yang Y., Li X., Dong W., Guan M., Zhang Q, & Xue Y. (2021). High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease. Frontiers in Pharmacology, 12, 741087.
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2

Measuring Serum and Urine Aldosterone, Potassium, and Sodium

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Serum and urine aldosterone levels were measured with commercial ELISA kits (E-EL-0070c, Elabscience, Wuhan, Hubei, China) according to the manufacturer’s descriptions. The colorimetric method was used for analysis of potassium and sodium. Serum and urine potassium and sodium levels were, respectively, measured with a commercial Potassium Assay kit and Sodium Assay kit (C003-1-1 and C002-1-1, Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China) following the manufacturer’s descriptions.
Wu L.L., Zhang Y., Li X.Z., Du X.L., Gao Y., Wang J.X., Wang X.L., Chen Q., Li Y.H., Zhu G.Q, & Tan X. (2022). Impact of Selective Renal Afferent Denervation on Oxidative Stress and Vascular Remodeling in Spontaneously Hypertensive Rats. Antioxidants, 11(5), 1003.
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3

Noninvasive Blood Pressure Monitoring

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The systolic-, diastolic- and mean blood pressures were measured at indicated weeks by a computerized noninvasive tail-cuff manometry system (BP-2010A; Softron Bio technology, Beijing, China). The five measured blood pressure values from each rat were averaged. To ensure the accuracy of blood pressure measurement, the rats were trained for one week before the experiments, in order for the rats to be acclimated to the procedure.
The rats were placed in metabolic cages for urine collection. The 24-hour-urine volumes and sodium excretions were obtained at the indicated times. The rats were acclimatized in the metabolic cages for at least 2 days before collecting the urine. Sodium concentrations in the urine samples were measured by Sodium Assay Kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). In addition, serum selenium levels were measured by inductively coupled plasma mass spectrometry (Agilent 7900, Agilent Technologies, CA, USA).
Lei L., Zhang F., Huang J., Zhou X., Yan H., Chen C., Zheng S., Si L., Jose P.A., Zeng C, & Yang J. (2023). Selenium deficiency causes hypertension by increasing renal AT1 receptor expression via GPx1/H2O2/NF-κB pathway. Free radical biology & medicine, 200, 59-72.
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