Mouse leptin elisa

Manufactured by R&D Systems

The Mouse Leptin ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse leptin levels in cell culture supernatants, serum, and plasma. The assay utilizes a monoclonal antibody specific for mouse leptin that is pre-coated onto a microplate. Samples and standards are pipetted into the wells, and any leptin present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for mouse leptin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells, and color develops in proportion to the amount of leptin bound. The intensity of the color is measured using a spectrophotometric microplate reader.

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Lab products found in correlation

Autokit glucose, by Fujifilm (2 mentions) Heparinized tubes, by Thermo Fisher Scientific (2 mentions) Mouse insulin elisa, by Mercodia (2 mentions)

Close spelling variants of this product

Mouse/rat leptin ELISA kit Mouse Leptin Quantikine ELISA Kit Mouse Leptin DuoSet ELISA Kit Mouse/Rat Leptin Quantikine ELISA Kit Mouse/Rat Leptin Quantikine ELISA Quantikine® ELISA Mouse/Rat Leptin Immunoassay Mouse/rat leptin enzyme-linked immunosorbent assay (ELISA) kit Mouse Leptin R DuoSet ELISA Mouse Leptin DuoSet ELISA Mouse/Rat Leptin Quantikine ELISA Kit (SMOB00)

2 protocols using mouse leptin elisa

Plasma Metabolite Analysis in Fed Mice

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Approximately 20 µl of whole blood was obtained by submandibular bleed at 9–10 a.m. from mice in a fed state. Blood was collected on ice using heparinized tubes (Fisherbrand) and plasma was isolated by centrifugation for 20 min at 2000 × g at 4 °C, aliquoted, and frozen at −80 °C until time of assay. Plasma leptin concentration was measured using mouse leptin ELISA (R&D) with sensitivity of 22 pg/ml. Plasma insulin concentration was measured using mouse insulin ELISA (Mercodia) and plasma glucose was measured with Autokit Glucose (WAKO).

Skowronski A.A., Shaulson E.D., Leibel R.L, & LeDuc C.A. (2021). The postnatal leptin surge in mice is variable in both time and intensity and reflects nutritional status. International Journal of Obesity (2005), 46(1), 39-49.

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Plasma Biomarker Measurements in Mice

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Approximately 20 ul of whole blood was obtained by submandibular bleed at 9–10 am from mice in a fed state. Blood was collected on ice using heparinized tubes (Fisherbrand) and plasma was isolated by centrifugation for 20 min at 2,000 × g at 4°C, aliquoted, and frozen at −80°C until time of assay. Plasma leptin concentration was measured using mouse leptin ELISA (R&D) with sensitivity of 22 pg/ml. Plasma insulin concentration was measured using mouse insulin ELISA (Mercodia) and plasma glucose was measured with Autokit Glucose (WAKO).

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