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Bio plex cell lysis kit solution

Manufactured by Bio-Rad
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The Bio-Plex cell lysis kit solution is a laboratory product designed to facilitate the extraction and preparation of cellular contents for downstream analysis. It is a buffer-based solution that disrupts cell membranes and releases intracellular components, including proteins, nucleic acids, and other biomolecules.

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Lab products found in correlation

Pfrs2α, by Cell Signaling Technology (2 mentions) Bio plex phosphoprotein detection system, by Bio-Rad (2 mentions) Phospho erk1 2, by Cell Signaling Technology (2 mentions) Phospho akt, by Cell Signaling Technology (2 mentions) Erk1 2, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

Bio-Plex (286 citations) Bio-Plex kit (41 citations) Cell lysis kit (33 citations) Cell lysis solution (2 citations) Lysis solution (2 citations)

2 protocols using bio plex cell lysis kit solution

1

Analyzing Akt and FGF Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
To analyze Akt signaling, mouse tissues were homogenized in Bio-Plex cell lysis kit solution (Bio-Rad) and lysates were analyzed for total and phospho-Akt following manufacturer’s instructions for the Bio-Plex phosphoprotein detection system (Bio-Rad). For in vitro FGF signaling assays, HEK293 cells were seeded in 6-well plates and grown to 70–80% confluence. Following overnight serum starvation in DMEM cells were treated with recombinant peptides for 15 min, washed in ice-cold PBS, and frozen in liquid nitrogen. Cells were lysed in sample buffer, sonicated, resolved on SDS-PAGE, and blotted to PVDF membranes for antibody detection. pFRS2α, ERK1/2, phospho-ERK1/2, Akt and phospho-Akt antibodies were purchased from Cell Signaling Technology.
Suh J.M., Jonker J.W., Ahmadian M., Goetz R., Lackey D., Osborn O., Huang Z., Liu W., Yoshihara E., van Dijk T., Havinga R., Fan W., Yin Y.Q., Yu R.T., Liddle C., Atkins A.R., Olefsky J.M., Mohammadi M., Downes M, & Evans R.M. (2014). Endocrinization of FGF1 produces a neomorphic and potent insulin sensitizer. Nature, 513(7518), 436-439.
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2

Analyzing Akt and FGF Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
To analyze Akt signaling, mouse tissues were homogenized in Bio-Plex cell lysis kit solution (Bio-Rad) and lysates were analyzed for total and phospho-Akt following manufacturer’s instructions for the Bio-Plex phosphoprotein detection system (Bio-Rad). For in vitro FGF signaling assays, HEK293 cells were seeded in 6-well plates and grown to 70–80% confluence. Following overnight serum starvation in DMEM cells were treated with recombinant peptides for 15 min, washed in ice-cold PBS, and frozen in liquid nitrogen. Cells were lysed in sample buffer, sonicated, resolved on SDS-PAGE, and blotted to PVDF membranes for antibody detection. pFRS2α, ERK1/2, phospho-ERK1/2, Akt and phospho-Akt antibodies were purchased from Cell Signaling Technology.
Suh J.M., Jonker J.W., Ahmadian M., Goetz R., Lackey D., Osborn O., Huang Z., Liu W., Yoshihara E., van Dijk T., Havinga R., Fan W., Yin Y.Q., Yu R.T., Liddle C., Atkins A.R., Olefsky J.M., Mohammadi M., Downes M, & Evans R.M. (2014). Endocrinization of FGF1 produces a neomorphic and potent insulin sensitizer. Nature, 513(7518), 436-439.
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