Log In Sign up
The largest database of trusted experimental protocols

Goat α lamin b1 sc 6217

Manufactured by Santa Cruz Biotechnology
Visit Supplier

Goat α-Lamin B1 (sc-6217) is a primary antibody that recognizes the Lamin B1 protein. Lamin B1 is a structural protein that is a component of the nuclear lamina, which provides mechanical support and organization to the cell nucleus. This antibody can be used in various immunological techniques to detect and study the Lamin B1 protein.

Automatically generated - may contain errors

Lab products found in correlation

Pvdf membrane, by Merck Group (2 mentions) Chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Ibright cl1000 imaging system, by Thermo Fisher Scientific (2 mentions) Benzonase, by Merck Group (2 mentions) Rat α ha, by Roche (2 mentions) Rabbit α hltf a300 230a, by Fortis Life Sciences (2 mentions) Mouse α ung2, by OriGene (2 mentions) Goat α rat hrp, by Jackson ImmunoResearch (2 mentions) Goat α mouse hrp, by Jackson ImmunoResearch (2 mentions) Goat α rabbit hrp, by Jackson ImmunoResearch (2 mentions)

Spelling variants of this product

Lamin B1 (108 citations) Sc-6217 (31 citations) Goat α-Lamin B1 (2 citations)

2 protocols using goat α lamin b1 sc 6217

1

Western Blot Analysis of DNA Repair Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in Laemmli buffer supplemented with 100 mM dithiothreitol (DTT) and benzonase (1:100, Sigma-Aldrich). Following denaturation at 65°C, samples were separated by SDS-PAGE and transferred to PVDF-membranes (Millipore) by TransBlot semi-dry transfer. Membranes were blocked in 5% (w/v) milk – PBS-T (0.2% Tween-20) and incubated with primary antibody in milk-PBS-T at 4°C overnight followed by incubation with HRP-conjugated secondary antibodies at room temperature (RT). Blots were developed using chemiluminescent substrates (Thermo Scientific) and visualised using either X-ray film or an iBright CL1000 imaging system (Thermo Fisher Scientific). The following antibodies were used for immunoblotting, listed by manufacturer: Primary antibodies: Abcam: Rabbit α-SLF2 (ab122480), rabbit α-VPRBP/DCAF1 (ab202587). Bethyl Laboratories: Rabbit α-HLTF (A300-230A). GeneTex: Rabbit α-SMC6 (GTX116832), rabbit α-NSMCE1 (GTX107136). NIH AIDS Reagent Program: Mouse α-Vif (#6459; (Simon et al., 1995 (link))). Origene: Mouse α-UNG2 (TA503563). Roche: Rat α-HA (11867423001). Santa Cruz: Goat α-Lamin B1 (sc-6217). Sigma-Aldrich: Mouse α-β-actin (A5316), rabbit α-ANKRD32/SLF1 (SAB2701555). Secondary antibodies: Jackson ImmunoResearch: Goat α-mouse-HRP (115-035-146), goat α-rabbit-HRP (115-035-144), and goat α-rat-HRP (115-035-143).
Dupont L., Bloor S., Williamson J.C., Cuesta S.M., Shah R., Teixeira-Silva A., Naamati A., Greenwood E.J., Sarafianos S.G., Matheson N.J, & Lehner P.J. (2021). The SMC5/6 complex compacts and silences unintegrated HIV-1 DNA and is antagonized by Vpr. Cell Host & Microbe, 29(5), 792-805.e6.
+ Open protocol
+ Expand
2

Western Blotting of DNA Repair Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in Laemmli buffer supplemented with 100 mM dithiothreitol (DTT) and benzonase (1:100, Sigma-Aldrich). Following denaturation at 65°C, samples were separated by SDS-PAGE and transferred to PVDF-membranes (Millipore) by TransBlot semi-dry transfer. Membranes were blocked in 5% (w/v) milk – PBS-T (0.2% Tween-20) and incubated with primary antibody in milk-PBS-T at 4°C overnight followed by incubation with HRP-conjugated secondary antibodies at room temperature (RT). Blots were developed using chemiluminescent substrates (Thermo Scientific) and visualised using either X-ray film or an iBright CL1000 imaging system (Thermo Fisher Scientific). The following antibodies were used for immunoblotting, listed by manufacturer: Primary antibodies: Abcam: Rabbit α-SLF2 (ab122480), rabbit α-VPRBP/DCAF1 (ab202587). Bethyl Laboratories: Rabbit α-HLTF (A300-230A). GeneTex: Rabbit α-SMC6 (GTX116832), rabbit α-NSMCE1 (GTX107136). NIH AIDS Reagent Program: Mouse α-Vif (#6459; (Simon et al., 1995 (link))). Origene: Mouse α-UNG2 (TA503563). Roche: Rat α-HA (11867423001). Santa Cruz: Goat α-Lamin B1 (sc-6217). Sigma-Aldrich: Mouse α-β-actin (A5316), rabbit α-ANKRD32/SLF1 (SAB2701555). Secondary antibodies: Jackson ImmunoResearch: Goat α-mouse-HRP (115-035-146), goat α-rabbit-HRP (115-035-144), and goat α-rat-HRP (115-035-143).
Dupont L., Bloor S., Williamson J.C., Cuesta S.M., Shah R., Teixeira-Silva A., Naamati A., Greenwood E.J., Sarafianos S.G., Matheson N.J, & Lehner P.J. (2021). The SMC5/6 complex compacts and silences unintegrated HIV-1 DNA and is antagonized by Vpr. Cell Host & Microbe, 29(5), 792-805.e6.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!