Recombinant human TNFα, IFNγ, TGFβ, IL-6 were purchased from Genentech Inc. (San Francisco, CA, USA). Human IL-1α was purchased from Sigma- Aldrich, (St. Louis, MO, USA). IL-7 and IL-15 were purchased from Peprotec (Rocky Hill, NJ). ALCAM-Fc, HER2-Fc were purchased from R&D Systems (Minneapolis, MN, USA).
Alcam fc
Manufactured by R&D Systems
Sourced in United States
ALCAM-Fc is a recombinant protein that consists of the extracellular domain of the human Activated Leukocyte Cell Adhesion Molecule (ALCAM) fused to the Fc region of human IgG1. ALCAM is a cell surface glycoprotein that mediates cell-cell adhesion.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human tnfα, by Genentech (2 mentions)
Interleukin 6 (il 6), by Genentech (2 mentions)
Her2 fc, by R&D Systems (2 mentions)
Ifn γ, by Genentech (2 mentions)
Human il 1α, by Merck Group (2 mentions)
Foetal calf serum fcs, by Merck Group (1 mentions)
Dmem ham s f 12 with l glutamine, by Merck Group (1 mentions)
Antimicrobial solution, by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Du145, by American Type Culture Collection (1 mentions)
Hfob1, by American Type Culture Collection (1 mentions)
Mda pca 2b, by American Type Culture Collection (1 mentions)
Pz hpv 7, by American Type Culture Collection (1 mentions)
Ca hpv 10, by American Type Culture Collection (1 mentions)
3 protocols using alcam fc
1
Cytokine and Receptor Protein Acquisition
2
Characterization of Prostate Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PC-3, LNCaP, VCaP, CAHPV-10, PZHPV-7, hFOB1.19, MDA-PCa-2b and DU-145 cell lines were purchased from the American Type Culture Collection (ATCC; Middlesex, UK). Human HECV endothelial cells were purchased from Interlab Cell Line Collection (ICLC; Genoa, Italy). PC-3, LNCaP, VCaP, HECV and hFOB1.19 cells were used in functional assays. PC-3, VCaP and HECV cells were grown in Dubecco’s Modified Eagle Medium (DMEM)/Ham’s F12 with L-glutamine (Sigma-Aldrich, Dorset, UK) and LNCaP cells were cultured in RPMI 1640 medium (Sigma-Aldrich, Dorset, UK). Human hFOB1.19 osteoblasts were maintained in DMEM/Ham’s F-12 without phenol red (Life Technologies, Paisley, UK) containing 0.3mg/ml G418 (Melford Laboratories, Suffolk, UK). All base mediums were supplemented with 10% foetal calf serum (FCS) (Sigma-Aldrich, Dorset, UK) and an antimicrobial solution (Sigma-Aldrich, Dorset, UK). Cells were cultured at 95% humidity, 5% CO2 and 37° C except hFOB1.19 which were cultured at 34° C.
HGF was a kind gift from Dr T Nakamura (Osaka University Medical School, Japan). Recombinant human ALCAM, comprising a fusion of ALCAM Trp28 - Ala526 and the human IgG Fc region (ALCAM-Fc chimera) was purchased from R&D systems (Abingdon, UK).
HGF was a kind gift from Dr T Nakamura (Osaka University Medical School, Japan). Recombinant human ALCAM, comprising a fusion of ALCAM Trp28 - Ala526 and the human IgG Fc region (ALCAM-Fc chimera) was purchased from R&D systems (Abingdon, UK).
3
Cytokine and Receptor Protein Acquisition
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