Cell migration was investigated using 5-µm transwell inserts for 24-well cell culture plates (Sarstedt). Briefly, 2 × 105 in vitro–expanded ILC2s in ILC2 medium were placed in the upper compartment. The well below was filled with ILC2 medium containing the recombinant chemokine CCL1, CCL8, CCL17, or CCL22 (all from BioLegend) at the indicated concentration or 5 µM of the activating CCR8 agonist ZK756326 (Selleckchem). After incubation for 2.5 h in a 37°C incubator, the migrated cells in the lower compartment were counted using a Neubauer chamber. The chemotaxis index was defined as the ratio of migrated cells to the respective stimulus and migrated cells without chemokines.
Ccl22
Manufactured by BioLegend
CCL22 is a chemokine that plays a role in the recruitment and migration of immune cells, particularly T cells and dendritic cells, to sites of inflammation. It functions by binding to the CCR4 receptor.
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Lab products found in correlation
2 protocols using ccl22
1
Transwell assay for ILC2 migration
2
Chemokine Receptor CCR4 Modulation
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The following drugs were used: recombinant CCL17 and CCL22 were purchased from BioLegend. The CCR4 antagonist C 021 (CAS 864289–85-0) was purchased from MilliporeSigma.
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