Chonblock blocking buffer

RSV A2 DsCav1 and hMPV F 130BV, as well as all the human reference mAbs were diluted at 100 μg/mL in Octet buffer (PBS + 0.02% Tween20, 0.1% BSA) and loaded onto HIS1K Biosensors. Vaccinated mice serum samples from each vaccination group were pooled and serial diluted (1/10, 1/100, and 1/1000) in ChonBlock blocking buffer (25% ChonBlock buffer diluted in Octet buffer) (Chondrex Inc.), while pooled serum from PBS vaccinated mice was diluted at 1/10 in ChonBlock blocking buffer. The loaded biosensors were immersed into wells containing diluted serum samples for 300 s, the non-specific binding signal was then removed by immersing the sensors in Octet buffer. Following this, biosensors were immersed into wells containing 100 µg/mL of a human reference mAb that binds to different epitopes for 300 s. Percent inhibition of pooled mice serum was calculated by (1 – max signal of the vaccinated serum/max signal of the naive serum) × 100%.