An automated fluorescence cell counter (NanoEnTek, Seoul, Republic of Korea) was used to count stained cells. After treating the cells with hinokitiol for 48 h, they were harvested and suspended in 1 mL of phosphate-buffered saline (PBS). Following manufacture instruction, Accustain solution N (NanoEnTek, Republic of Korea) was then used to calculate the number of apoptotic cells, while Accustain solution T was used to calculate the total number of cells. The Accustain solutions are commonly used for the detection of apoptotic cells and can accurately distinguish between apoptotic and necrotic cells.
To perform cell counting, the cell suspension was mixed with the staining solution and loaded into the Accu-Chip 4X (NanoEnTek, Republic of Korea). This chip is a microfluidic device that is designed to count cells using a fluorescence-based detection method. The Accu-Chip 4X can count up to four samples simultaneously, allowing for a high-throughput analysis of multiple samples.
The Accu-Chip 4X was then loaded into the NanoEnTek automated fluorescence cell counter, and the cell counts were obtained. The equipment uses a combination of fluorescence and imaging technologies to count the cells and generate accurate and reliable results.
To perform cell counting, the cell suspension was mixed with the staining solution and loaded into the Accu-Chip 4X (NanoEnTek, Republic of Korea). This chip is a microfluidic device that is designed to count cells using a fluorescence-based detection method. The Accu-Chip 4X can count up to four samples simultaneously, allowing for a high-throughput analysis of multiple samples.
The Accu-Chip 4X was then loaded into the NanoEnTek automated fluorescence cell counter, and the cell counts were obtained. The equipment uses a combination of fluorescence and imaging technologies to count the cells and generate accurate and reliable results.