Two interferents, commercial lipid emulsion (Oliclinomel N-7, 1000 E (20%, Baxter Inc. Lessines, Belgium)) and the native ultralipemic material (NULM) prepared in-house, were used. To prepare NULM, a 100 mL serum pool was collected from approximately 40 residual lipemic serum samples (BD Vacutainer SST II Plus, 5 mL, Becton Dickinson, Franklin Lake, USA) with a triglyceride (TG) concentration > 22.6 mmol/L. The samples from patients who used IVLE or came from the intensive care unit were not included in the study to prevent IVLE contamination. Samples were not frozen, as freeze-thaw cycles would cause errors. They were stored at 2-8 °C for seven days by the stability of the lipids (20 (link)). Then, this pool was centrifuged three times at 45,000xg for 30 minutes in a Hanil Supra 21K (Hanil Scientific Inc., Gimpo, South Korea) refrigerated high-speed centrifuge. After each centrifugation, the supernatant lipid layer was collected carefully. Subsequently, only this lipid-rich portion underwent centrifugation in the following step. At the end of the third collection, we had 5mL of NULM.
Vacutainer sst 2 plus
Manufactured by BD
Sourced in United States
The BD Vacutainer SST II Plus is a blood collection tube used for the separation and collection of serum samples. It contains a gel barrier that separates the serum from the cellular components of the blood sample during centrifugation.
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Lab products found in correlation
6 protocols using vacutainer sst 2 plus
1
Preparation of Native Ultralipemic Material
2
Blood Serum Vitamin D Measurement
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Blood samples were drawn from an antecubital vein using a hypodermic needle (21G, BD Vacutainer® Safety-Lok™, #368654, Becton Dickinson AB, Stockholm, Sweden) and collected in 5 mL serum separation tubes (BD Vacutainer® SST II Plus, #367955, Becton Dickinson AB, Stockholm, Sweden), while the subject sat in a comfortable position. During visits 1, 2, 5, and 8, three blood samples were drawn, one of which served as a backup sample. The blood samples were turned upside down 5-6 times manually to ensure that the sample was thoroughly mixed with the clot activator in the serum separation tube. The samples were then left for a minimum of 30 minutes to allow clotting before being centrifuged at 3000 rpm for 10 minutes at 20°C. For each serum separation tube, the supernatant serum was transferred to a transfer tube. The transfer tubes were kept at -80°C until further analysis. Serum was analyzed for 25(OH)D and 1,25(OH) 2 D by chemiluminescence enzyme immunoassay using an IDS-iSYS Fully Automated Immunoassay System.
3
Serum Collection for Newly Diagnosed Antibody-Positive Myasthenia Gravis
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Sera from newly diagnosed AChR antibody positive MG patients with extraocular muscle involvement were collected in BD Vacutainer SST II Plus plastic serum tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). These samples were left to clot at room temperature for 4 h and centrifuged in a swinging bucket rotor centrifuge at 1900 g for 10 min at 4 °C. Serum was then removed, aliquoted and stored at -20 °C. Importantly, the patients selected for sera donation had generalized MG with substantial extraocular muscle involvement and were treatment-naïve (newly diagnosed or one individual known with MG but defaulted treatment for ⩾ 5 months). Control sera were collected from normal controls.
4
Serum SDF-1 Protein Quantification by ELISA
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Serum SDF-1 protein levels were determined using an ELISA kit (MCX120; R&D Systems), following the manufacturer’s protocol. Under general anesthesia, 5 mL of blood was drawn via cardiac puncture at the time of sacrifice. Serums were separately collected using a serum separator blood collecting tube (BD Vacutainer® SST™ II Plus; BD, Franklin Lakes, NJ, USA), centrifuged at 1000 g for 10 min, and stored at − 80 °C until analysis. The ELISA was performed in duplicate for each sample. The colorimetric density of the developed microplate was determined using a Multiskan Sky Microplate Spectrophotometer and SkanIt software (Thermo Fisher Scientific, Waltham, MA, USA) at 450 nm. Wavelength corrections were performed by subtracting the readings at 540 nm or 570 nm from those at 450 nm for optical imperfection correction.
5
Postpartum Blood Sampling Protocol
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Fasting blood samples (4-5 cc) were taken from participants in 5-mL gelled tubes (BD Vacutainer® SST II Plus) and immediately transferred to the laboratory using a cool box. The specimens were centrifuged to separate serums from the blood cells and transferred to microtubes (2 ccs) for storing at -80 • C. Postpartum blood samples were collected from 39 pregnant women at the time of delivery to one day after that. The informed consent form was received from each subject. Besides, Shiraz University of Medical Sciences Ethics Committee approved the study protocol by the code of 'I.R.SUMS.REC.1398.140".
6
Monocyte isolation from SSc and healthy subjects
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Twenty two patients who fulfilled the American College of Rheumatology criteria according to LeRoy for the classification of SSc [15] were obtained from Wroclaw Medical University.
This study was approved by the local ethics committee (approval no. 335/2014) SSc patients provided fully informed written consent. Their clinical characteristics and treatment are summarised in Table 1 and2, respectively. Twenty one donors with no history of autoimmune disease were included as healthy control. The blood from healthy controls (HC) was collected from a local blood donor centre or directly from healthy volunteers. The blood was collected in EDTA-coated tubes from HC and SSc patients. Peripheral blood mononuclear cells (PBMC) were separated from whole red blood cells as described elsewhere [1] . To isolate monocytes fraction, PBMC were incubated with anti-CD14+ beads (Miltenyi-Biotec) according to the manufacturer's protocol. Serum samples from HC, RA and SSc patients were collected in serum separation tubes (BD Vacutainer® SST II Plus), aliquoted and frozen at -20°C.
This study was approved by the local ethics committee (approval no. 335/2014) SSc patients provided fully informed written consent. Their clinical characteristics and treatment are summarised in Table 1 and2, respectively. Twenty one donors with no history of autoimmune disease were included as healthy control. The blood from healthy controls (HC) was collected from a local blood donor centre or directly from healthy volunteers. The blood was collected in EDTA-coated tubes from HC and SSc patients. Peripheral blood mononuclear cells (PBMC) were separated from whole red blood cells as described elsewhere [1] . To isolate monocytes fraction, PBMC were incubated with anti-CD14+ beads (Miltenyi-Biotec) according to the manufacturer's protocol. Serum samples from HC, RA and SSc patients were collected in serum separation tubes (BD Vacutainer® SST II Plus), aliquoted and frozen at -20°C.
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