MTS-8-MTS-cross-linked RD-ABD1/F-actin samples were resolved on 9% SDS-PAGE and transferred to nitrocellulose. Western blotting was performed using anti-actin (1:2000; #MA5–11869, ThermoFisher Scientific, Waltham, MA) and anti-PLS3 (1:1000; #SAB2700266, Millipore-Sigma, Burlington, MA) primary antibodies followed by anti-mouse (#A4416) and anti-rabbit (#A0545) secondary antibodies conjugated to horseradish peroxidase (both are from Millipore-Sigma and used at 1:10,000). WesternBright Sirius chemiluminescent horseradish peroxidase substrate (Advansta, San Jose, CA) was used for signal detection in Omega Lum G imaging system (Aplegen, Pleasanton, CA) using OmegaLum G Image Capture software v.2.1.
Westernbright sirius chemiluminescent horseradish peroxidase substrate
Manufactured by Advansta
WesternBright Sirius is a chemiluminescent horseradish peroxidase (HRP) substrate. It is used for the detection of HRP-labeled proteins in Western blotting applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using westernbright sirius chemiluminescent horseradish peroxidase substrate
1
SDS-PAGE and Western Blot Analysis
2
Immunoblotting of Actin and PLS3
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MTS-8-MTS-cross-linked RD-ABD1/F-actin samples were resolved on 9% SDS-PAGE and transferred to nitrocellulose. Western blotting was performed using anti-actin (1:2000; #MA5-11869, ThermoFisher Scientific) and anti-PLS3 (1:1000; #SAB2700266, Millipore-Sigma, Burlington, MA) primary antibodies followed by anti-mouse (#A4416) and anti-rabbit (#A0545) secondary antibodies conjugated to horseradish peroxidase (both are from Millipore-Sigma and used at 1:10,000).
WesternBright Sirius chemiluminescent horseradish peroxidase substrate (Advansta, San Jose, CA) was used for signal detection in Omega Lum G imaging system (Aplegen, Pleasanton, CA).
WesternBright Sirius chemiluminescent horseradish peroxidase substrate (Advansta, San Jose, CA) was used for signal detection in Omega Lum G imaging system (Aplegen, Pleasanton, CA).
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