Recombinant human α syn

Phosphate buffer saline (PBS), trypsin, DMEM/F-12, and antibodies against CD68 were purchased from Thermo Fisher Scientific (Waltham, MA). Fetal bovine serum (FBS) was bought from Atlas Biologicals (Fort Collins, CO). Antibiotic-antimycotic was purchased from Sigma (St. Louis, MO). Recombinant human α-syn was purchased from Anaspec (Fremont, CA). Antibodies against anti-goat Iba1 were purchased from Abcam (Waltham, MA). Dako antibodies against glial fibrillary acidic protein (GFAP) were bought from Agilent (Santa Clara, CA). Antibodies against myelin basic protein (MBP) and microtubule associated protein 2 (MAP2) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Cy2- and Cy5-conjugated antibodies were obtained from Jackson Immuno-Research Laboratories (West Grove, PA).

Dulbecco’s Modified Eagle Medium (DMEM) medium was purchased from Mediatech (Washington, DC) and fetal bovine serum (FBS) was obtained from Atlas Biologicals (Fort Collins, CO). Antibiotic-antimycotic was purchased from Sigma-Aldrich (St. Louis, MO). Recombinant human α-syn was purchased from Anaspec (Fremont, CA). Rabbit anti-tyrosine hydroxylase (TH) antibody was purchased from Pel-Freeze biologicals (Rogers, AR) and mouse anti-TH antibody was procured from Immunostar (Hudson, WI). Mouse α-syn antibody was purchased from BD Bioscience (San Jose, CA) (Supplementary Table S1). Cy2- and Cy5-conjugated antibodies were obtained from Jackson Immuno-Research Laboratories (West Grove, PA).