Apc cd11b antibody

The right untreated tumor was extracted, immersed in digestion solution and incubated at 37 °C for 1 h. The cell suspension was then filtered through a 40 µm filter to remove debris and cell clumps. The cells were washed with PBS and resuspended. The cell concentration was adjusted to 1 × 10 7 /ml in l00 µl for examination. The corresponding surface antibodies (1:200) were added and incubated on ice in the dark for 20 min for surface antibody staining. After surface antibody staining was completed, 60 µl of cytokine fixation solution was added and fixed on ice in the dark for 20 min. The cells were washed twice with cytokine permeabilization buffer, and cytokine staining antibodies (1:200) were prepared with cytokine permeabilization buffer. The cells were incubated on ice in the dark for 25 min for cytokine staining. Anti-mouse FITC CD45 Antibody, PerCP/ Cyanine5.5 CD4 Antibody, PE/Cyanine7 CD8a Antibody, PE FOXP3 Antibody, FITC Ly-6G/Ly-6C (Gr-1) Antibody, APC CD11b Antibody (Biolegend, USA) were used. Detection was performed on a Beckman Coulter (USA) flow cytometer, and the percentages of immune cells and immunosuppressive cells in right tumor tissues were analyzed by CytExpert software.