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Anti mcpyv lt cm2b4

Manufactured by Santa Cruz Biotechnology
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Anti-MCPyV LT (Cm2B4) is a mouse monoclonal antibody that recognizes the large T antigen (LT) of Merkel cell polyomavirus (MCPyV). The antibody is intended for research use only, and its core function is to detect and study the MCPyV LT protein.

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Lab products found in correlation

Spinning disk confocal microscope, by Nikon (1 mentions) Anti kap1, by Abcam (1 mentions) Mouse alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Anti pkap1 s824, by Abcam (1 mentions) Alexa fluor 555, by Thermo Fisher Scientific (1 mentions) Anti vinculin v9131, by Merck Group (1 mentions) Anti β tubulin tub 2.1, by Merck Group (1 mentions)

2 protocols using anti mcpyv lt cm2b4

1

Quantifying Phosphorylation of Kap1 in nHDF Cells

Cited 1 time
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A total of 4 × 104 nHDF cells were seeded on gelatin-coated coverslips and analyzed by immunofluorescence (36 (link)) using the following antibodies: anti-MCPyV LT (Cm2B4; Santa Cruz) (1:500), anti-Kap1 (catalog no. 10483; Abcam) (1:1,000), anti-pKap1 S824 (catalog no. 70369; Abcam) (1:1,000), anti-mouse Alexa Fluor 488 (Life Technologies; catalog no. A-11001), anti-mouse Cy5 (A10523), and anti-rabbit Alexa Fluor 555 (A-21428). Staining was analyzed on a Nikon spinning-disk confocal microscope with a 20× nonconfocal lens objective or a 100× confocal lens objective. Quantification of phosphorylation intensities was performed by applying the Fiji software package and ImageJ, evaluating 100 cells each.
Siebels S., Czech-Sioli M., Spohn M., Schmidt C., Theiss J., Indenbirken D., Günther T., Grundhoff A, & Fischer N. (2020). Merkel Cell Polyomavirus DNA Replication Induces Senescence in Human Dermal Fibroblasts in a Kap1/Trim28-Dependent Manner. mBio, 11(2), e00142-20.
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2

Immunoblotting of Merkel Cell Polyomavirus Proteins

Cited 1 time
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Immunoblotting was performed as previously described [32 (link)]. The following antibodies were used in this study: anti-MCPyV-LT (CM2B4; Santa Cruz Biotechnology, Dallas, TX, USA), anti-MCPyV-TA (2T2 hybridoma supernatant; [34 (link)]), anti-AURKA (1G4; Cell Signaling, Danvers, MA, USA), anti-β-tubulin (TUB 2.1; Sigma-Aldrich, Taufkirchen, Germany) and anti-vinculin (V9131; Sigma Aldrich).
Houben R., Alimova P., Sarma B., Hesbacher S., Schulte C., Sarosi E.M., Adam C., Kervarrec T, & Schrama D. (2023). 4-[(5-Methyl-1H-pyrazol-3-yl)amino]-2H-phenyl-1-phthalazinone Inhibits MCPyV T Antigen Expression in Merkel Cell Carcinoma Independent of Aurora Kinase A. Cancers, 15(9), 2542.
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