Immunocytochemistry was performed on whole tadpoles and subsequently the hearts were dissected, mounted in 12 μl CyGEL Sustain (biostatus) and viewed using Zeiss LSM5-Pascal or LSM710 confocal microscopes. Confocal images of whole hearts captured 2 μm deep optical sections. Both the ventricle myocardial wall and also deeper trabecular layers were assessed by scanning different depths. Images of cardiomyocytes and myofibrils were 1 μm optical sections, at a depth 1–2 μm below the outer (apical) myocardial surface. Antibodies used were Adprhl1, Myosin A4.1025 (DSHB) and phospho-Histone H3(Ser10) 3H10 (Sigma), along with fluorescent dye-conjugated secondary antibodies (Jackson ImmunoResearch). Atto 633-conjugated phalloidin (Sigma) stained actin filaments. Dying cells were visualized with the ApopTag® Red In Situ Apoptosis Detection kit (Sigma).
Phospho histone h3 ser10 3h10
Manufactured by Merck Group
Phospho-Histone H3(Ser10) 3H10 is a monoclonal antibody that specifically recognizes histone H3 phosphorylated at serine 10. It is a useful tool for the detection and quantification of this post-translational modification, which is associated with various cellular processes such as mitosis and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710, by Zeiss (4 mentions)
Lsm 5 pascal, by Zeiss (2 mentions)
Atto 633 conjugated phalloidin, by Merck Group (2 mentions)
Fluorescent dye conjugated secondary antibodies, by Jackson ImmunoResearch (2 mentions)
Apoptag red in situ apoptosis detection kit, by Merck Group (2 mentions)
Cygel sustain, by Biostatus (2 mentions)
2 protocols using phospho histone h3 ser10 3h10
1
Imaging Cardiomyocytes in Developing Tadpoles
2
Immunocytochemistry of Tadpole Hearts
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Immunocytochemistry was performed on whole tadpoles and subsequently the hearts were dissected, mounted in 12 µl CyGEL Sustain (biostatus) and viewed using Zeiss LSM5-Pascal or LSM710 confocal microscopes. Confocal images of whole hearts captured 2 µm deep optical sections. Both the ventricle myocardial wall and also deeper trabecular layers were assessed by scanning different depths. Images of cardiomyocytes and myofibrils were 1 µm optical sections, at a depth 1-2 µm below the outer (apical) myocardial surface. Antibodies used were Adprhl1, Myosin A4.1025 (DSHB) and phospho-Histone H3(Ser10) 3H10 (Sigma), along with fluorescent dyeconjugated secondary antibodies (Jackson ImmunoResearch). Atto 633-conjugated phalloidin (Sigma) stained actin filaments. Dying cells were visualized with the ApopTag ® Red In Situ Apoptosis Detection kit (Sigma).
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