P450 glotm cyp3a assay

Manufactured by Promega

Sourced in United States

The P450-GloTM CYP3A assay is a luminescence-based assay that measures the activity of the CYP3A enzyme. It provides a simple and sensitive method for evaluating the metabolism of compounds by the CYP3A enzyme.

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Lab products found in correlation

Luciferin ipa, by Promega (2 mentions) Triiodothyronine t3, by Merck Group (1 mentions) 3 methylcholanthrene 3 mc, by Merck Group (1 mentions)

2 protocols using p450 glotm cyp3a assay

Enzymatic Activity Assays for CYP2E1, CYP3A, and CYP1A2

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CYP2E1 activity was determined by the rate of oxidation of p-nitrophenol (PNP) to p-nitrocatechol in the presence of NADPH and O2. The activity was determined using the following equation: PNP activity (in nanomoles per minute per milligram of protein) = D₅₄₆/9.53/0.2/60/7.1 × 10³. CYP3A activity was measured using a P450-GloTM CYP3A assay with luciferin-IPA (Promega) according to the instruction. CYP1A2 activity was measured by the rate of 7-ethoxyresorufin O-deethylation (EROD) in the presence of NADPH and O2. EROD was measured by HPLC as previously described in Pastrakuljic A et al^{41 (link)}.

Wang C., Xu W., Zhang Y., Huang D, & Huang K. (2018). Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity. Cell Death & Disease, 9(8), 819.

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Thyroid Hormone and CYP3A Interaction

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Triiodothyronine (T3) and 3-methylcholanthrene (3-MC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Thyroxine (T4) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Dexamethasone (DEX) was purchased from Nacalai tesque (Kyoto, Japan). The P450-Glo TM CYP3A assay with Luciferin-IPA and Luciferin-MultiCYP were purchased from Promega (Fitchburg, WI, USA).

Mori J., Sanoh S., Kashiwagi K., Hanada H., Shigeta M., Suzuki K.T., Yamamoto T., Kotake Y., Sugihara K., Kitamura S., Kashiwagi A, & Ohta S. (2017). Developmental changes in drug-metabolizing enzyme expression during metamorphosis of Xenopus tropicalis. The Journal of toxicological sciences, 42(5).

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