Orca hr amtv542

Manufactured by Hamamatsu Photonics

Sourced in Japan

The ORCA-HR Amtv542 is a scientific imaging camera developed by Hamamatsu Photonics. It is designed for high-resolution, low-noise imaging applications. The camera features a scientific-grade CMOS sensor and advanced electronics to capture detailed images with high sensitivity and speed.

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Lab products found in correlation

Jem 100cx, by JEOL (2 mentions)

2 protocols using orca hr amtv542

Ultrastructural Analysis of NB4 Cells

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The NB4 (CT and NTAL-KD) cells were treated overnight or not with 10 µM chloroquine. The cells were fixed in 2% glutaraldehyde in 0.1 M sodium phosphate buffer at pH 7.4 (PB) for 2 h. The specimens were post-fixed in PB buffer containing 1% OsO₄ for 2 h, and dehydrated in an ethanol series (30%, 50%, 70%, 80%, 95%, and 100%), for 10 min each, followed by 2 times with propylene oxide, for 5 min each. They were then infiltrated with propylene oxide and epoxy resin (V/V = 1:1), embedded with EPON 812 epoxy resin, DDSA, DMP-30, and MNA resin, and then aggregated for 24–48 h at 60 °C. Ultra-thin sections (60–70 nm) were cut with a diamond knife and stained with uranyl acetate and lead citrate. Sections were examined with a TEM (Jeol, Jem 100cx, Tokyo, Japan). Pictures were taken and converted to digital files (Hamamatsu, ORCA-HR Amtv542, Hamamatsu City, Japan).

Thomé C.H., Ferreira G.A., Pereira-Martins D.A., dos Santos G.A., Ortiz C.A., de Souza L.E., Sobral L.M., Silva C.L., Scheucher P.S., Gil C.D., Leopoldino A.M., Silveira D.R., Coelho-Silva J.L., Traina F., Koury L.C., Melo R.A., Bittencourt R., Pagnano K., Pasquini R., Nunes E.C., Fagundes E.M., Gloria A.B., Kerbauy F.R., Chauffaille M.D., Keating A., Tallman M.S., Ribeiro R.C., Dillon R., Ganser A., Löwenberg B., Valk P., Lo-Coco F., Sanz M.A., Berliner N., Faça V.M, & Rego E.M. (2020). NTAL is associated with treatment outcome, cell proliferation and differentiation in acute promyelocytic leukemia. Scientific Reports, 10, 10315.

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Ultrastructural Analysis of LG Tissues

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LG tissues from the three groups that were fixed for TEM were rinsed in a 0.1 M phosphate buffer, dehydrated through a graded ethanol series, rinsed in acetone, and embedded in an Embed 812 (EM Sciences). Sections (60-70 nm) were cut with a diamond knife and stained for 25 min each in 2% uranyl acetate and 5 min in Reynolds' lead citrate. Sections were examined with a TEM (Jeol, Jem 100cx, Tokyo, Japan). Pictures were taken and converted to digital files (Hamamatsu, ORCA-HR Amtv542, Hamamatsu City, Japan). The intra cellular organelles, including secretory vesicles and nuclei, were eva luated in each group.

Dias A.C., Batista T.M., Roma L.P., Módulo C.M., Malki L.T., Dias L.C., Alves M., Reinach P.S., Carneiro E.M, & Rocha E.M. (2015). Insulin replacement restores the vesicular secretory apparatus in the diabetic rat lacrimal gland. Arquivos brasileiros de oftalmologia, 78(3).

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