Cells were cultured with either vehicle, and/or VitD3 (final concentration 10−7 M; Sigma), and/or RORα/γ modulator SR-1078 (5 µM; Merck-Millipore, Watford, UK). Transfection in the presence of 1 µM Stealth siRNA duplexes (Life Technologies; Supplemental Table 1 ) was performed by electroporation (Amaxa Nucleofector Lonza, Slough, UK). After 24, 48, or 72 hours, cells were subjected to trypan blue viability assay, cytospin for hematoxylin and eosin stain (Sigma), MTS assay for total viable cell number (Promega, Southampton, UK), Annexin V/propidium iodide stain for apoptosis quantitation (BD Biosciences, Oxford, UK), and/or BrdU proliferation assay as per the manufacturer’s protocols (BD Biosciences). Statistical significance was determined by Student t test.
Sr 1078
Manufactured by Merck Group
Sourced in United Kingdom, United States
The SR-1078 is a laboratory equipment product manufactured by the Merck Group. It is designed to perform precise and controlled operations in a laboratory setting. The core function of the SR-1078 is to facilitate the execution of standardized laboratory procedures and experiments, but a detailed description of its specific capabilities is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Brdu proliferation assay, by BD (1 mentions)
Vitd3, by Merck Group (1 mentions)
Stealth sirna duplexes, by Thermo Fisher Scientific (1 mentions)
Amaxa nucleofector, by Lonza (1 mentions)
Melatonin, by Merck Group (1 mentions)
Sr1001, by Merck Group (1 mentions)
Aa 861, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
2 protocols using sr 1078
1
Modulation of Cell Viability and Apoptosis
2
Activation of HSC by Melatonin and Analogs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melatonin (Sigma-Aldrich), SR1078 (Merck Millipore, Billerica, MA, USA), SR1001 (Sigma-Aldrich), and AA861 (Sigma-Aldrich) were dissolved in DMSO (Merck) and diluted with medium for final concentrations. Treatment concentrations were 10 lM Melatonin, 10 lM SR1078, 10 lM SR1001, and 10 lM AA861, unless stated otherwise. Isolated HSC were treated 4 hr after plating. Every day medium was changed and supplied with fresh Melatonin, SR1078, SR1001, AA861, or DMSO as control group. Activated HSC were trypsinized and recultured for 3 days. Treatment was started 4 hr after plating.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!