Lambda 35 or 25

A stock solution of CT-DNA was prepared in PBS buffer, which gave a ratio of UV absorbances at 260 nm and 280 nm (A260/A280) of ca. 1.8-1.9, indicating that the DNA was sufficiently free of protein and the concentration was determined by the UV absorbance at 260 nm (ε = 6600 M -1 cm -1 ) [15] .
The UV-Vis spectra were obtained on a Perkin-Elmer Lambda 35 or 25 double beam spectrophotometer, using 1.0 cm path-length quartz cuvettes (3.0 mL). Fluorescence measurements were run on a RF-1501 PC spectrofluorometer (Shimadzu, Japan). The fluorescence spectra were recorded in the range 550-750 nm upon excitation at 527 nm in all cases. The excitation and emission bandwidths were both 10 nm.

A stock solution of CT-DNA was prepared in PBS buffer, which gave a ratio of UV absorbances at 260 nm and 280 nm (A 260 / A 280 ) of ca. 1.8-1.9, indicating that the DNA was sufficiently free of protein and the concentration was determined by the UV absorbance at 260 nm (ε = 6600 M -1 cm -1 ). 26 The UV-Vis spectra were obtained on a Perkin-Elmer Lambda 35 or 25 double beam spectrophotometer, using 1.0 cm path-length quartz cuvettes (3.0 mL). Fluorescence measurements were performed on a RF-1501 PC spectrofluorometer (Shimadzu, Japan). The fluorescence spectra were recorded in the range of 550-750 nm upon excitation at 527 nm in all cases. The excitation and emission bandwidths were both 10 nm.