Acrodisc 25 mm syringe filter

All solvents and chemicals purchased from commercial sources were of analytical grade or better and were used without further purification. DX600, NODAGA‐DX600, and DOTA‐DX600 were custom synthesized by ChinaPeptides Co., Ltd (Shanghai, China) or CSBio (San Diego, California). Sep‐Pak Accell Plus QMA and Sep‐Pak C18‐Light cartridges were purchased from Waters (Ireland). Acrodisc 25 mm syringe filter (0.22 µm) was purchased from Pall Corporation (USA). The product was analyzed by radio‐ high performance liquid chromatography (HPLC) (1200, Agilent, USA) equipped with γ detector (Flow‐count, Bioscan, Washington. D.C., USA), using a C18 column (Eclipse Plus C18, 4.5 × 250 mm, 5µm, Agilent, USA). The product purity was also determined using Radio‐TLC (AR 2000, Bioscan, USA) after radiolabeling. The PET/CT imaging studies of small animals were performed on the Mira PET/CT of PINGSENG Healthcare Inc. (Shanghai, China), or microPET R4 rodent scanner (Siemens) and analyzed by ASIProVM. The Clinical PET/CT scans were obtained on a Biograph mCT Flow 64 scanner (Siemens, Erlangen, Germany) with unenhanced low‐dose CT. ⁶⁸Ga‐HZ20 PET/MRI was performed on a hybrid 3.0T PET/MR scanner (uPMR790, UIH, Shanghai, China) in female volunteers.

The lipid peroxidation level of mites after the exposure to fenpropathrin was investigated using Lipid Peroxidation MDA Assay Kit (Beyotime, Beijing, China) according to the manufacturer's instruction. After exposure, mites were homogenized in phosphate buffer (0.1 M, pH 7.5) on ice, and the homogenate was centrifuged at 15,000 g for 15 min. The pellets were discarded and the supernatant was filtered through Acrodisc 25 mm Syringe Filter (Pall Corporation, Port Washington, NY, USA). The filtered supernatant was used for the measurement of lipid peroxidation level, while corresponding protein content was measured according to the method of Bradford using bovine serum albumin as a standard (Bradford, 1976 (link)).