Bacterial strains and plasmids used in this study are shown in Table 1 . A. actinomycetemcomitans (ATCC 43718), F. nucleatum subsp. nucleatum (ATCC 25586), P. gingivalis (ATCC 33277), T. forsythia (ATCC 43037), and T. denticola (ATCC 33521) were obtained from American Type Culture Collection (Manassas, VA, USA). Bacterial cultures were grown under anaerobic growth conditions at 37 °C using an anaerobic growth chamber AS‐580 (Anaerobe Systems, Morgan Hill, CA, USA) supplied with anaerobic gas (90% N2, 5% H2, and 5% CO2). F. nucleatum and P. gingivalis were cultured in brain heart infusion, T. forsythia was cultured using brain heart infusion supplemented with 2.5 mM N‐acetylmuramic acid (Sigma, St. Louis, MO, USA). A. actinomycetemcomitans and T. denticola were cultured in MTGE‐anaerobic enrichment broth (Anaerobe Systems).
Mtge anaerobic enrichment broth
Manufactured by Anaerobe Systems
MTGE‐anaerobic enrichment broth is a pre-reduced, anaerobically sterilized culture medium designed for the isolation and cultivation of anaerobic microorganisms. It provides the necessary nutrients and growth factors to support the growth of a wide range of anaerobic bacteria.
Automatically generated - may contain errors
Lab products found in correlation
P gingivalis, by American Type Culture Collection (1 mentions)
T forsythia, by American Type Culture Collection (1 mentions)
N acetylmuramic acid, by Merck Group (1 mentions)
F nucleatum, by Merck Group (1 mentions)
P gingivalis, by Merck Group (1 mentions)
T denticola, by American Type Culture Collection (1 mentions)
As 580, by Anaerobe Systems (1 mentions)
2 protocols using mtge anaerobic enrichment broth
1
Anaerobic Cultivation of Oral Bacteria
2
CD8+ T cell Activation by Bacterial Lysates
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L. goodfellowii (HM-12; BEI Resources), B. theta (provided by A. Goodman and A. Wexler, Yale University, New Haven, CT), B. dorei (BEI Resources), and L. reuteri (BEI Resources) were grown in MTGE Anaerobic Enrichment Broth (Anaerobe Systems) overnight. For heat-killed bacteria, the bacterial pellet was resuspended in sterile 1× PBS and heated at 65°C for 60 min. The bacterial lysates were then used to stimulate the NY8.3 CD8+ T cells followed by intracellular cytokine (ICC) analysis.
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