Additionally, the proliferation of U-2OS cells was evaluated by counting the number of viable cells with Celigo (Nexcelom Bioscience, Lawrence, MA, USA). Cells were seeded at 1,500 cells/well in 96-well plates and incubated at 37°C with 5% CO2. Each group was prepared in triplicate. From the second day, images of cells with green fluorescent protein (GFP) were taken and cells were counted each day using Celigo (Nexcelom Bioscience). Cell growth was observed continuously for 5 days, and cell growth curves were generated.
Celigo
Manufactured by Revvity
Sourced in United States, United Kingdom
The Celigo is a cell imaging and analysis system designed for efficient cell-based assays. It provides automated imaging and quantitative analysis of cell samples in multiwell plates. The Celigo captures images of cells and quantifies various cellular parameters such as cell count, viability, and morphology.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (4 mentions)
Propidium iodide, by Thermo Fisher Scientific (4 mentions)
96 wounding replicator, by V&P Scientific (3 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions)
Mitotracker red, by Thermo Fisher Scientific (3 mentions)
Mtt reagent, by Genview (2 mentions)
Microplate reader, by Tecan (2 mentions)
Multiskan fc, by Thermo Fisher Scientific (2 mentions)
Lsm 880 confocal microscope, by Zeiss (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Celltiter glo luminescent cell viability assay, by Promega (1 mentions)
Alexa fluor 488 conjugated goat anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade, by Thermo Fisher Scientific (1 mentions)
Annexin 5 apc kit, by Thermo Fisher Scientific (1 mentions)
96 well plate, by Corning (1 mentions)
Dragon green beads, by Bangs Laboratories (1 mentions)
Matrigel, by Corning (1 mentions)
Celltiter blue, by Promega (1 mentions)
Ultra low attachment 96 well plate, by Corning (1 mentions)
Sytox green, by Thermo Fisher Scientific (1 mentions)
61 protocols using celigo
1
Proliferation Assay of U-2OS Cells
2
Cell Growth Quantification in Kidney Tumor Cells
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Cell growth was examined using Celigo (Nexcelom, Lawrence, MA) with EGFP. Kidney tumour cells in each experimental group in logarithmic growth phase were made into cell suspensions using trypsin. Cells were then seeded in 96‐well plates at a density of 1 × 103 or 2 × 103 cells/well. Each group had three plates, and the culture system was 100 μl per plate. The cells were cultured at 37℃. From the second day after plating, the plates were scanned once a day by Celigo (Nexcelom, Lawrence, MA) for 5 days.
3
Lentiviral Knockdown of CCNI2 in RKO Cells
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RKO cells, infected with lentivirus‐shCCNI2 s, were trypsinized, resuspended and counted 2000 cells that were seeded into 96‐well plate. Then the cells were cultured in an incubator with 5% CO2 at 37°C. After 24 h, Celigo (Nexcelom Bioscience, Lawrence, Massachusetts, USA) was used to scan the 96‐well plate at the same time for five consecutive days to obtain the scanning images, and Image J was used to count the cells in the scanning images.
4
Cell Migration Assay Protocol
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A wound healing assay was used to determine the migration of the cells. A total of 5×104 cells were seeded into 96-well plates and grown to 80-90% confluency. A scratch line was made in the cell monolayer by a pipette tip. Cells were further cultured for different periods of time as indicated. The migrated cells were quantified using Celigo (Nexcelom, USA), and the migration rate was calculated.
5
Quantitative Wound Healing Assay
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Transfected cells were plated, and scratches were made in wells in the central portion of the lower end of a 96-well plate (VP scientific, Shanghai, China). Serum-free medium was used to gently rinse the cells 2–3 times, low-serum medium was added, and photos were taken under a fluorescence microscope. Cells were cultured at 37°C in a 5% CO2 incubator, and the plates were scanned with a Celigo (Nexcelom, USA) at the indicated times to record the degree of healing. The areas with migratory cells were analyzed on the Celigo.
6
Cell Proliferation and ERK5 Inhibition Assay
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BT549 and MDA‐MB‐231 were continuously cultured to a cell density of up to 2000 cells per well. Next day, the cells were counted by Celigo (Nexcelom) at the same time every day for consecutive 5 days. In addition, the cell proliferation ability was tested after the cells were treated with BIX02189. Notably, BIX02189 (ChemeGen, Cat. No. C101208) is a potent and selective extracellular signal–regulated kinase 5 (ERK5) inhibitor with an IC50 of 1.5 nM.20 Subsequently, the number of green fluorescent cells in each scanning orifice plate was accurately calculated, and the cell proliferation curve was drawn. After 14 days, the cells were first fixed with 4% paraformaldehyde for 60 minutes and then stained with Giemsa solution for 20 minutes; finally, cell clones were photographed.
7
Lentivirus-mediated PSMC2 Knockdown Impacts Cell Proliferation
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Lentivirus-infected (shCtrl, shPSMC2) DU 145 and PC-3 cells were seeded at a 96-well plate with 2000 cells per well for culturing. The plate was continuously detected by Celigo (Nexcelom, Lawrence, MA, USA) for 5 days at the same time. Cell proliferation rate was analyzed.
8
Cell Growth Curve Analysis
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AGS and MGC-803 cells were transfected with lentivirus and cultured in 96-well plates at a density of 2000 cells per well. The cells were counted at 24, 48, 72 and 96 h by Celigo (Nexcelom) and the cell growth curve was plotted for the 5 days.
9
Clonogenic Assay of HL-60 Cells
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HL-60 cells were treated with NUC-7738 for 72 hrs prior to seeding 30 x 103 cells onto MethoCultTM enriched medium (Stemcell Technologies, UK, H4435) and colonies were recorded using Celigo (Nexcelom Bioscience) after 14 days incubation at 37°C and 5% CO2 incubation.
10
Measuring Cell Viability Using ATP and Image Cytometry
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Two different assays were used to measure cell viability. First, CellTiter-Glo Luminescent Cell Viability Assay (Promega) was used to measure ATP generated by metabolically active cells as an indicator of cell viability. Second, in a non-ATP dependent manner, the number of cells were assessed from a 96-well plate using an in situ image cytometer (Celigo, Nexcelom Bioscience, Lawrence, MA). Assays were done using the manufacturer's protocol. Briefly, 1-2 × 104 cells per well were cultured in 96-well plates in the absence or presence of the drugs as indicated. Treatment was done for 72 hours unless otherwise indicated. At the end of the treatment, 100 μl of CellTiter-Glo reagent was added. Luminescence was recorded in a Multiskan FC (ThermoFisher) plate reader luminometer with an integration time of 1 s per well. All wells in both assays were done in triplicates.
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