For the differentiation of 3T3-L1 cells, stock solutions of differentiation reagents were prepared. Insulin (Sigma-Aldrich, Sydney, Australia) was prepared as a 2 mg/mL solution in 0.01 M aqueous hydrochloric acid (AJAX, Themo Fisher Scientific, Melbourne, Australia) and syringe-filtered. Dexamethasone (Sigma-Aldrich, Sydney, Australia) was prepared as a freezer stock solution of 10 mM stock solution in chromatography grade ethanol (Merck Pty Ltd., Melbourne, Australia), and was further diluted to a working stock solution of 1 mM in phosphate buffered saline, pH 7.4 (PBS; Thermo Fisher Scientific, Melbourne, Australia). 3-Iso-butyl-1-methylxanthine (IBMX; Sigma-Aldrich) was prepared as a 50 mM stock solution in dimethyl sulfoxide (DMSO; Sigma-Aldrich).
Ammonium acetate solution (0.1 M) was prepared via the dissolution of Ammonium acetate (Merck Pty Ltd., Melbourne, Australia) in Milli-Q® ultrapure deionised water (Merck Millipore, Melbourne, Australia). The solution was syringe-filtered prior to use.
Ammonium acetate solution (0.1 M) was prepared via the dissolution of Ammonium acetate (Merck Pty Ltd., Melbourne, Australia) in Milli-Q® ultrapure deionised water (Merck Millipore, Melbourne, Australia). The solution was syringe-filtered prior to use.