Snu 5

GC cell lines SNU‐5, HGC‐27, N87 and SGC‐7901 and the normal gastric mucosal cell line GES1 were purchased from ATCC (Manassas VA). RPMI1640 culture media (GIBCO‐BRL; Thermo Fisher Scientific, Waltham, MA) containing 100 U/ml streptomycin/penicillin and 10% fetal bovine serum (FBS) (Hyclone, USA) were placed in a thermostatic incubator containing 5% CO₂ at saturated humidity at 37°C.

The human GC cell lines AGS and NCI-N87 and the human normal gastric mucosal cell line GES-1 were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The human GC cell lines Hs-746T and SNU-719 were purchased from Procell (Wuhan, China). The human GC cell line SNU-5 was obtained from ATCC. Cells were cultured in RPMI-1640 medium (Gibco, Grand Island, NY, United States) supplemented with 10% fetal bovine serum (FBS) (NBCS) (PAA Laboratories, Inc., Pasching, Austria) at 37°C in an atmosphere of 5% CO2.

GC samples were collected from patients who underwent surgery at Fudan University Shanghai Cancer Center between 2012 and 2013. The protocol was approved by the Clinical Research Ethics Committee of Fudan University, and the research was carried out according to the provisions of the Helsinki Declaration of 1975. All samples were obtained with the informed consent of the patients. The human GC cell line AGS (ATCC® CRL-1739™), SNU-1 (ATCC® CRL-5971™), SNU-5 (ATCC® CRL-5973™), SNU-16 (ATCC® CRL- 5974™), NCI-N87 (ATCC® CRL-5822™), and KATO III (ATCC® HTB-103™) were maintained in DMEM containing 10% fetal bovine serum. All cell lines were maintained in media containing penicillin (100 IU/ml) and streptomycin (100 mg/ml) at 37°C with 5% CO2. The miRNA mimics and inhibitors were purchased from Ambion (Austin, TX, USA).

The human gastric cancer cell lines, HSC-39, HSC-43, HSC-59, HSC-60, HSC-64, HSC-44PE, 58As9, 58As1, and 44As3, have been described previously [40 (link),41 (link),42 (link),43 (link),44 (link)]. The human gastric cancer cell lines, MKN1, MKN7, MKN74, NUGC-4, KATO-III, MKN45, IM95, and human lung cancer cell line EBC-1 were obtained from the JCRB Cell Bank (Osaka, Japan). The human gastric cancer cell lines, NCI-N87 and SNU-5, human lung cancer cell line A549, and human mesothelial cell line Met5A were obtained from ATCC (Rockville, MD, USA). The human gastric cancer cell lines, GCIY, ECC12, H-111-TC, GSU, and KE-97, were provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan (Ibaraki, Japan). These cells were maintained in RPMI 1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum, 10 U/mL of penicillin, and 10 µg/mL of streptomycin at 37 °C in a humidified atmosphere containing 5% CO₂. Mycoplasma contamination was tested using a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).