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4-CQA is a chemical compound that functions as a standard analytical reference material. It is commonly used in research applications to identify and quantify the presence of this compound in various samples.

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2 protocols using 4 cqa

1

Procurement of Bioactive Compounds

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Puerarin (≥99.71%), 3-CQA (≥99.37%), 4-CQA (≥99.39%), 5-CQA (≥99.07%) and EGCG were purchased from Chengdu Must Biotechnology Co., Ltd (Chengdu, China). Methanol and acetonitrile for HPLC were obtained from Scharlau Co., Ltd (Barcelona, Spain). AA was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). All other chemicals were of analytical grade and obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
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2

Quantification of Chlorogenic Acid Derivatives

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Methanol (HPLC grade) was obtained from Thermo Fisher Scientific (Pittsburgh, PA, USA). Formic acid was bought from Dimka Pure (Richmond Hill, NY, USA). Purified water was purchased from the Hangzhou Wahaha Group (Hangzhou, China). Others reagents and chemicals were all of analytical grade. The reference standards of CA, 3-CQA, 4-CQA, 5-CQA, 1,3-diCQA, 1,5-diCQA, 4,5-diCQA, 1,3,5-tirCQA were purchased from Chengdu Must Bio-technology Co.; Ltd. (Chengdu, China). The chemical structures of the eight reference compounds were determined to be higher than 98%.
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